中国临床药理学杂志2024，Vol.40Issue(1)：42-46,5.DOI:10.13699/j.cnki.1001-6821.2024.01.009
枸杞多糖调控AMPK/ULK1自噬途径对高渗诱导角膜上皮细胞凋亡的影响
Influences of Lycium barbarum polysaccharide on apoptosis of corneal epithelial cells induced by hypertonicity through regulation of AMPK/ULK1 autophagy pathway
摘要
Abstract
Objective To investigate the influences of Lycium barbarum polysaccharide on apoptosis of corneal epithelial cells induced by hypertonicity through regulating adenosine monophosphate activated protein kinase(AMPK)/uncoordinated 51-like kinase 1(ULK1)autophagy pathway.Methods The ophthalmoxerosis cell model was established by osmotic pressure of 500 mOsm·L-1 on corneal epithelial cells.They were divided into model group,positive control group(0.3％sodium hyaluronate eye drops),inhibitor group(1 mg·mL-1 Lycium barbarum polysaccharide+10 μmol·L-1compound C),experimental-L,-H groups(0.5,1.0 mg·mL 1 Lycium barbarum polysaccharide),and normal cultured CRL-11135 cells were taken as blank group(no treatment was performed).Cell apoptosis was detected by flow cytometry.Autophagy was detected by MDC staining.Western blot was used to detect the expressions of p-AMPK/AMPK and p-ULK1/ULK1.Results The apoptosis rates of experimental-L,experimental-H,positive control,inhibitor,model and blank groups were(26.47±2.13)％,(13.68±2.21)％,(12.54±2.16)％,(18.73±2.12)％,(37.56±3.25)％and(6.35±2.14)％;the relative contents of autophagosomes were(59.63±8.14)％,(89.89±9.04)％,(90.31±9.13)％,(62.75±7.26)％,(43.11±6.45)％and(100.00±0.00)％;p-AMPK/AMPK were 0.45±0.07,0.64±0.08,0.66±0.06,0.53±0.04,0.34±0.05 and 0.87±0.06;p-ULKl/ULKl were 0.54±0.06,0.75±0.05,0.77±0.03,0.65±0.04,0.46±0.04 and 0.92±0.08,respectively.The above indexes in experimental-L,-H groups and positive control group were significantly different from those in model group(all P＜0.05);the above indexes in inhibitor group were significantly different from those in experimental-H group(all P＜0.05).Conclusion Lycium barbarum polysaccharide can inhibit the apoptosis of corneal epithelial cells induced by hypertonicity by activating the AMPK/ULK1 autophagy pathway.关键词
枸杞多糖/眼干燥症/腺苷酸活化蛋白激酶/不协调的51类激酶1/角膜上皮细胞/高渗/凋亡Key words
Lycium barbarum polysaccharide/ophthalmoxerosis/adenosine monophosphate activated protein kinase/uncoordinated 51-like kinase 1/corneal epithelial cell/hypertonicity/apoptosis分类
医药卫生引用本文复制引用
田雯雯,段志学,蒲建中,王晶,律鹏..枸杞多糖调控AMPK/ULK1自噬途径对高渗诱导角膜上皮细胞凋亡的影响[J].中国临床药理学杂志,2024,40(1):42-46,5.基金项目
甘肃省科技计划基金资助项目(21JR7RA422) （21JR7RA422）
