中国临床药理学杂志2024，Vol.40Issue(1)：47-51,5.DOI:10.13699/j.cnki.1001-6821.2024.01.010
钩藤碱对甲基苯丙胺依赖SH-SY5Y细胞模型及miR-375-3p/Elavl4表达的影响
Effects of rhynchophylline on methamphetamine-dependent SH-SY5Y cells model and expression of miR-375-3p/Elavl4
摘要
Abstract
Objective To investigate the effects of rhynchophylline on methamphetamine-dependent SH-SY5Y cells model and microRNA-375-3p(miR-375-3p)/embryonic lethal abnormal vision drosophila-like 4(Elavl4)expression.Methods A methamphetamine-dependent cell model by maximum safe dose induction was established.The cells were divided into normal group(complete culture medium),control group(complete culture medium+400 μmol·L-1 rhynchophylline incubated for 48 h),model group(complete culture medium+100 μmnol·L-1 methamphetamine incubated for 48 h)and experimental group(complete culture medium+400 μmol·L-1 rhynchophylline incubated for 15 min,then 100 μmol·L-1 methamphetamine incubated for 48 h).The cyclic adenosine monophosphate(cAMP)and 5-hydroxytryptamine(5-HT)expression were detected by enzyme-linked immunosorbent assay(ELISA),miR-375-3p expression was detected by quantitative real time polymerase chain reaction(qRT-PCR),and Elavl4 expression was detected by Western blot.Results The cAMP expression levels of normal group,control group,model group and experimental group were(6.33±0.93),(6.57±1.12),(10.89±1.03)and(7.81±1.32)pmol·mg-1;5-HT were(682.46±17.32),(690.31±15.09),(510.11±27.67)and(649.99±21.42)pg·mL-1;miR-375-3p expression were 1.00±0.13,1.13±0.24,3.48±0.18 and 1.58±0.19;Elavl4 expression were 1.00±0.05,0.89±0.10,0.50±0.09 and 0.90±0.11,respectively.The differences between above indicators in model group and normal group were statistically significant(P＜0.01,P＜0.05);the differences between above indicators in experimental group and model group were statistically significant(P＜0.01,P＜0.05).Conclusion This study preliminarily established a methamphetamine-dependent cell model,and also found that rhynchophylline may regulate miR-375-3p/Elavl4 expression to antagonize methamphetamine addiction.关键词
甲基苯丙胺/药物依赖/钩藤碱/微小RNA-375-3p/胚胎致死异常视觉-样4蛋白Key words
methamphetamine/drug dependence/rhynchophylline/microRNA-375-3p/embryonic lethal abnormal vision drosophila-like 4分类
医药卫生引用本文复制引用
李汉成,曾茗,陈依玲,陈玲红,莫志贤..钩藤碱对甲基苯丙胺依赖SH-SY5Y细胞模型及miR-375-3p/Elavl4表达的影响[J].中国临床药理学杂志,2024,40(1):47-51,5.基金项目
国家自然科学基金资助项目(81873030) （81873030）
广东省重点领域研发计划基金资助项目(2021B0707010008) （2021B0707010008）
广东省科技厅农村科技特派员课题资助项目(KTP20200176) （KTP20200176）
广东省教育厅青年创新人才基金资助项目(2023kQNCX090) （2023kQNCX090）
肇庆市科技指导类基金资助项目(2023040316003) （2023040316003）
肇庆学院博士科研启动基金资助项目(220003) （220003）
肇庆学院科研基金资助项目(QN202224) （QN202224）
肇庆学院大学生创新创业训练计划基金资助项目(S202310580075,S202310580070) （S202310580075,S202310580070）
