中国临床药理学杂志2024，Vol.40Issue(1)：67-71,5.DOI:10.13699/j.cnki.1001-6821.2024.01.014
四磨汤调控NLRP3/Caspase-1/GSDMD信号通路对功能性消化不良大鼠十二指肠微炎症的影响
Effect of Simo decoction on the regulation of NLRP3/Caspase-1/GSDMD signal pathway on duodenal microinflammation in rats with functional dyspepsia
摘要
Abstract
Objective To investigate the effects of Simo decoction on duodenal microinflammation and NOD-like receptor thermal protein domain associated protein 3(NLRP3)/cysteinyl aspartate-specific proteinase-1(Caspase-1)/gasdermin D(GSDMD)signaling pathway in rats with functional dyspepsia(FD).Methods The FD model was established by multifactorial method.SD rats were randomly divided into normal group,model group(FD model),positive control group(gavage administration of 0.305 mg·kg-1 mosapride injection)and experimental-H,-M,-L groups(gavage administration of 5.62,2.81,1.40 g·kg-1 Simo decoction).Small intestinal advancement rate and gastric emptying rate was determined;the levels of interleukin(IL)-1 β and IL-18 in serum were determined by enzyme linked immunosorbent assay(ELISA);the protein expression of NLRP3 and GSDMD in duodenal tissue was detected by Western blotting.Results The gastric emptying rates of normal,model,positive control and experimental-H,-M,-Lgroupswere(58.34±5.72)％,(29.16±8.37)％,(48.77±6.10)％,(48.35±6.04)％,(48.20±3.49)％and(39.24±4.20)％;the small intestinal propulsion rates were(82.01±7.55)％,(41.95±9.53)％,(64.61±10.18)％,(75.04±9.76)％,(60.58±7.13)％and(45.89±7.40)％;serum IL-1 β expression were(12.86±0.88),(43.73±4.60),(18.84±0.86),(24.61±1.57),(19.14±0.77)and(29.04±0.72)pg·mL-1;IL-18 expressions were(95.00±3.74),(170.60±8.78),(108.50±3.05),(118.90±3.45),(99.90±8.70)and(141.00±3.71)pg·mL-1;the relative expression levels of NLRP3 proteins were 0.32±0.02,0.84±0.05,0.42±0.03,0.48±0.02,0.61±0.04 and 0.62±0.05;the relative expression levels of GSDMD proteins were 0.34±0.05,0.93±0.06,0.35±0.03,0.52±0.02,0.53±0.06 and 0.55±0.05,respectively.Compared with the normal group,the above indexes in the model group have statistical significance;compared with the model group,the above indexes in the experimental-H group and the positive control group also have statistical significance(P＜0.01 or P＜0.05).Conclusion Simo decoction can effectively improve the general condition and duodenal microinflammation in FD rats,and the mechanism may be related to the inhibition of duodenal NLRP3/Caspase-1/GSDMD signaling pathway.关键词
四磨汤/功能性消化不良/十二指肠/炎症/核苷酸结合寡聚化结构域样受体蛋白3/胱天蛋白酶1/消皮素D信号通路Key words
Simo decoction/functional dyspepsia/duodenum/inflammation/NOD-like receptor thermal protein domain associated protein 3/cysteinyl aspartate-specific proteinase-1/gasdermin D signal pathway分类
医药卫生引用本文复制引用
刘琴,蔺晓源,阳凌峰,罗倩,韩运宗,陈思清,张海月,周姝,周赛男..四磨汤调控NLRP3/Caspase-1/GSDMD信号通路对功能性消化不良大鼠十二指肠微炎症的影响[J].中国临床药理学杂志,2024,40(1):67-71,5.基金项目
湖南省自然科学基金科卫联合基金资助项目(2022JJ70031) （2022JJ70031）
湖南省中医重点专科基金资助项目 （）
湖南省中医药科研计划重点基金资助项目(2021203) （2021203）
湖南中医药大学中医学一流学科开放基金重点项目(4901020000200222) （4901020000200222）
