中国临床药理学杂志2024，Vol.40Issue(1)：77-81,5.DOI:10.13699/j.cnki.1001-6821.2024.01.016
橙皮苷对雌激素和孕激素诱导雌性大鼠乳腺增生的抑制作用
Inhibitory effects of hesperidin on estrogen-and progestin-induced mammary hyperplasia in female rats
摘要
Abstract
Objective To investigate the therapeutic effect of hesperidin(Hsp)on mammary gland hyperplasia(MGH)rats and the possible mechanism.Methods Sixty female rats were randomly divided into blank,model,control and experimental-L,-M,-H groups.Except for blank group,all other groups were injected intramuscularly with estrogen and progestin to induce MGH model in rats.The experimental-L,-M and-H groups were given 25,50,100 mg·kg-1 Hsp by gavage.The control group was given 5 mg·kg-1 tamoxifen by gavage.The blank and control groups were given an equal volume of 0.9％NaCl by gavage.Six groups of rats were administered once a day for 30 days.After the administration,the height and diameter of nipples and the uterine coefficient were measured.The serum sex hormone content was detected using enzyme-linked immunosorbent assay,the levels of oxidative stress indicators were detected using corresponding kits,and the expressions of related proteins were detected using Western blot.Results The nipple diameters of rats in experimental-M and-H groups,control group,model group and blank group were(1.41±0.20),(1.18±0.21),(1.23±0.20),(1.75±0.13)and(1.02±0.06)mm;the nipple heights were(1.62±0.24),(1.41±0.19),(1.47±0.16),(1.87±0.32)and(1.23±0.14)mm;the uterine indexes were(0.55±0.08),(0.37±0.05),(0.41±0.04),(0.72±0.11)and(0.34±0.03)mg·g-1;the relative expression levels of estrogen receptor alpha(ERα)were 0.54±0.05,0.13±0.02,0.13±0.03,0.89±0.08 and 0.10±0.02;the relative expression levels of progesterone receptor(PR)were 0.43±0.03,0.08±0.01,0.87±0.07,0.20±0.04 and 0.14±0.03;the phosphorylated-extracellular-regulated kinase 1/2(ERK1/2)/ERK1/2 were 0.50±0.03,0.06±0.02,0.83±0.05,0.09±0.04 and 0.04±0.01,respectively.Compared with model group,the differences of above indexes in the experimental-M,-H groups and control group were statistically significant(all P＜0.05).Conclusion Hsp could reduce nipple diameter,height,and uterine coefficient,regulate sex hormone levels,resist oxidative stress,and inhibit the expression of ERα,PR and p-ERK1/2 proteins in rats.关键词
橙皮苷/雌激素/乳腺增生/超氧化物歧化酶/细胞外调节激酶1/2Key words
hesperidin/estrogen/mammary hyperplasia/superoxide dismutase/extracellular-regulated kinase 1/2分类
医药卫生引用本文复制引用
杨阳,杨晓芬,黄培森,冷婕,杨凡,李永佳..橙皮苷对雌激素和孕激素诱导雌性大鼠乳腺增生的抑制作用[J].中国临床药理学杂志,2024,40(1):77-81,5.基金项目
四川省中医药管理局中医药科研专项基金资助项目(2021MS485) （2021MS485）
