维库溴铵下调lncRNA FGD5-AS1对胃癌细胞HGC-27增殖和凋亡特性的影响OACSTPCD
Effects of vecuronium bromide down-regulation of lncRNA FGD5-AS1 on the proliferation and apoptosis of gastric cancer cell HGC-27
目的 探讨维库溴铵对胃癌细胞恶性生物学行为的影响及分子机制.方法 将胃癌细胞HGC-27分为对照组、低剂量实验组、中剂量实验组、高剂量实验组、si-NC 组、si-FGD5-AS1 组、pcDNA-FGD5-AS1 组、pcDNA 组、高剂量+pcDNA组、高剂量+pcDNA-FGD5-AS1组.对照组常规培养,低、中、高剂量实验组分别用5、10、20 μmol·mL-1维库溴铵处理,si-FGD5-AS1组和si-NC组分别转染lncRNA FGD5-AS1干扰表达载体及其阴性对照质粒,pcD-NA-FGD5-AS1组和pcDNA组分别转染lncRNA FGD5-AS1过表达载体及其阴性对照质粒,高剂量+pcDNA-FGD5-AS1组和高剂量+pcDNA组分别转染lncRNA FGD5-AS1过表达载体及其阴性对照质粒后用20 μmol·mL-1维库溴铵处理.用四甲基偶氮唑盐(MTT)比色法检测细胞活性,用流式细胞术检测细胞凋亡情况,用实时荧光定量聚合酶链反应(RT-qPCR)检测FGD5-AS1表达水平.结果 对照组、高剂量实验组、si-NC组、si-FGD5-AS1组、pcDNA组、pcDNA-FGD5-AS1 组、高剂量+pcDNA 组、高剂量+pcDNA-FGD5-AS1 组的细胞活性分别为 1.31±0.07、0.58±0.03、1.31±0.06、0.51±0.03、1.29±0.08、1.68±0.15、0.59±0.03 和 1.16±0.06,细胞凋亡率分别为(6.49±0.44)％、(23.52±0.98)％、(6.42±0.44)％、(26.75±0.97)％、(6.72±0.38)％、(2.56±0.19)％、(23.56±1.04)％和(11.65±0.47)％,lnc RNA FGD5-AS1 表达水平分别为 1.00±0.05、0.37±0.02、0.99±0.05、0.21±0.02、1.00±0.03、2.98±0.12、0.38±0.02 和 0.87±0.05.高剂量实验组的上述指标与对照组比较,si-FGD5-AS1组的上述指标与si-NC组比较,pcDNA-FGD5-AS1组的上述指标与pcDNA组比较,高剂量+pcDNA-FGD5-AS1组的上述指标与高剂量+pcDNA组比较,差异均有统计学意义(均P＜0.05).结论 维库溴铵可能通过下调lnc RNA FGD5-AS1抑制HGC-27细胞增殖,促进细胞凋亡.
Objective To investigate the effect of vecuronium bromide on the malignant biological behavior of gastric cancer cells and its molecular mechanism.Methods Gastric cancer cells HGC-27 were divided into control group,experimental-L,-M,-H groups,si-NC group,si-FGD5-AS1 group,pcDNA-FGD5-AS1 group,pcDNA group,experimental-H+pcDNA group,experimental-H+pcDNA-FGD5-AS1 group.The control group was cultured conventionally;experimental-L,-M,-H groups were treated with 5,10 and 20μmol·mL-1 vecuronium bromide,respectively;si-FGD5-AS1 group and the si-NC group were transfected with lncRNA FGD5-AS1 interference expression vector and negative control plasmid,respectively;pcDNA-FGD5-AS1 group and pcDNA group were transfected with lncRNA FGD5-AS1 overexpression vector and negative control plasmid,respectively;lncRNA FGD5-AS1 overexpression vector and negative control plasmid were transfected into HGC-27 cells in the experimental-H+pcDNA-FGD5-AS1 group and experimental-H+pcDNA group,and then treated with 20 μmuol·mL-1 vecuronium bromide.Methyl thiazolyl tetrazolium(MTT),flow cytometry and real-time fluorescent quantitative polymerase chain reaction(RT-qPCR)were applied to dectect cell viability,apoptosis and lncRNA FGD5-AS1 expression.Results The cell activity of control group,experimental-L,-M,-H groups,si-NC group,si-FGD5-AS1 group,pcDNA group,PCDNA-FGD5-AS1 group,experimental-H+pcDNA group and experimental-H+PCDNA-FGD5-AS1 group were 1.31±0.07,0.58±0.03,1.31±0.06,0.51±0.03,1.29±0.08,1.68±0.15,0.59±0.03 and 1.16±0.06;the apoptosis rates were(6.49±0.44)％,(23.52±0.98)％,(6.42±0.44)％,(26.75±0.97)％,(6.72±0.38)％,(2.56±0.19)％,(23.56±1.04)％and(11.65±0.47)％;the expression levels of lncRNA FGD5-AS1 were 1.00±0.05,0.37±0.02,0.99±0.05,0.21±0.02,1.00±0.03,2.98±0.12,0.38±0.02 and 0.87±0.05,respectively.The above indexes were compared with the control group and experimental-H group,those in the si-FGD5-AS1 group were compared with the si-NC group,those in the pcDNA-FGD5-AS1 group were compared with the pcDNA group,and those in the experimental-H+pcDNA-FGD5-AS1 group were compared with the experimental-H+pcDNA group,the differences were statistically significant(all P＜0.05).Conclusion Vecuronium bromide may inhibit the proliferation of HGC-27 cells and promote cell apoptosis by down-regulating lncRNA FGD5-AS1.
付滨;张科;刘星;代传兴;陈祖棋;邱德亮
成都医学院第二附属医院核工业四一六医院麻醉科,四川成都 610000成都市第一人民医院麻醉科,四川成都 610000四川大学华西医院龙泉医院麻醉科,四川成都 610000
药学
维库溴铵长链非编码RNA含有5个PH结构域的反义RNA1胃癌增殖凋亡
vecuronium bromidelong non-coding RNA contains five PH domains antisense RNAlgastric cancerproliferationapoptosis
《中国临床药理学杂志》 2024 (002)
205-209 / 5
四川省中医药信息学会关于舒适化医疗(新晨基金)专项科研课题基金资助项目(20200119)
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