中国临床药理学杂志2024，Vol.40Issue(2)：220-224,5.DOI:10.13699/j.cnki.1001-6821.2024.02.014
鸢尾素在阿霉素心肌病中的保护作用和机制
Protective role and mechanistic exploration of Irisin in doxorubicin induced-cardiomyopathy
摘要
Abstract
Objective To study the protective effect of Irisin in doxorubicin(Dox)induced-Cardiomyopathy and its possible mechanism.Methods AC 16 cells were used to construct Dox injury model and divided into control group(AC 16 cells were cultured with complete medium),Irisin group(AC16 cells were treated with 10 ng·L-1 Irisin for 24 h),Dox group(AC 16 cells were treated with 4 μmol·L-1 Dox for 24 h),Dox+Irisin group(AC 16 cells were pretreated with 10 ng·L-1 Irisin for 2 h,and then treated with 4 pmol·L-1 Dox for 24 h).Cell counting kit-8(CCK-8),terminal deoxynucleotidyl transferase-mediated nick end labeling(TUNEL)and lactate dehydrogenase(LDH)were used to detect the proliferation,apoptosis and mortality of AC 16 cells.Western blot was used to detect the expression levels of nuclear factor-κB(NF-κB)signaling pathway and apoptotic factors B-cell lymphoma-2(Bcl-2),Bcl-2-associated X protein(Bax)and caspase-9 protein.Mito-Tracker Red CMXRos probe was used to detect mitochondrial membrane potential.Results In the contrl group,Irisin group,Dox group,Dox+Irisin group,the rate of apoptosis were(0.97±0.09)％,0,(42.80±6.70)％,(11.74±1.79)％;the expression of Bax protein were 0.85±0.01,0.36±0.02,1.15±0.07,0.37±0.11;the expression of caspase-9 protein were 0.52±0.02,0.59±0.03,1.11±0.02,0.67±0.08;the expression of Bcl-2 protein were 1.01±0.04,1.05±0.25,0.43±0.02 and 0.99±0.30;the probability of mitochondrial damage were(0.02±0.01)％,(0.5±0.15)％,(38.6±2.39)％,(1.58±0.54)％.The difference of the above indexes between the contrl group and the Dox group were statistically significant(all P＜0.05);the difference between Dox group and Dox+Irisin group were statisically significant(all P＜0.05).Conclusion Irisin could reduce the expression level of Bax,caspase-9,p-NF-κB,and p-mTOR caused by Dox,increase the expression level of Bcl-2,ameliorate the myocardial damage caused by Dox,and reduce cardiotoxicity.关键词
鸢尾素/阿霉素/心肌损伤/细胞核因子-κB信号通路Key words
irisin/doxorubicin/cardiotoxicity/nuclear factor-κB signal pathway分类
医药卫生引用本文复制引用
金宇鸽,李松森,王皓,郭彩茹,张兵兵,陈浩杰,班亚鑫,梁如冰..鸢尾素在阿霉素心肌病中的保护作用和机制[J].中国临床药理学杂志,2024,40(2):220-224,5.基金项目
河南省重点研发与推广专项(科技攻关)基金资助项目(212102310810、222102310113) （科技攻关）
洛阳市公益性行业医疗卫生专项基金资助项目(2302038Y) （2302038Y）
