T2* mapping MR成像评价脊髓缺血再灌注后铁死亡的机制研究OACSTPCD

Objective:To examine the changes of iron deposition in the spinal cord by 7.0T T2* mapping MR imaging af-ter spinal cord ischemia-reperfusion(SCIR)and analyze the relationship between T2* and protein of ferroptosis,to reflect the mechanism of ferroptosis after SCIR.Methods:Forty-eight Wistar rats(male,280～300 g)were selected and randomly divided into sham group(n=8)and SCIR group(n=40).The SCIR group was divided into 6 h(n=8),12 h(n=8),24 h(n=8),48 h(n=8)and 72 h(n=8)subgroups.A 7.0T MR scan was performed after the modeling was completed.After scanning,immunohistochemical staining of family 11 member 2(SLC11A2),ferritin heavy chain 1(FTH1),glutathione peroxidase 4(GPX4)were performed.Analysis of variance and Tukey test were used to compare the differences in T2* values and protein expression levels in each group.Results:At 6 h after SCIR,the T2* value of the anterior spinal horn decreased significantly(P<0.001,Tukey test),reaching the lowest at 24 h(P<0.001,Tukey test),and the 48 h subgroup recovered(P<0.001).At 6 h after the SCIR injury,SLC11A2 expression showed a slight increase trend,while FTH1 expression was significantly reduced(P=0.007,Tukey test).At 24 h after the SCIR injury,the expression of SLC11A2,FTH1,and GPX4 were significantly decreased(P=0.031,P=0.009,P= 0.004,Tukey test).Conclusion:After SCIR injury,the T2* value,SLC11A2,FTH1,and GPX4 all show a transient decrease,and the T2* change is consistent with the GPX4 protein change,suggesting that T2* mapping can further reflect the mecha-nism of ferroptosis by detecting the changes in spinal iron deposition.