中国免疫学杂志2024，Vol.40Issue(1)：52-57,6.DOI:10.3969/j.issn.1000-484X.2024.01.006
艾蒿花粉诱导小鼠过敏性哮喘及其内在型研究
Mugwort pollen-induced mouse allergic asthma and endotyping
摘要
Abstract
Objective:To construct a mouse asthma model induced by mugwort pollen and to explore endotyping,providing methods for subsequent precision treatment.Methods:BALB/c mice were intraperitoneally injected with mugwort pollen extract(MPE)to sensitize,following MPE intranasal challenge to construct MPE allergic asthma murine model.Mice were randomly divided into PBS sensitization and PBS challenge(P-P),MPE sensitization and PBS challenge(M-P),MPE sensitization and MPE challenge model(M-M)groups.24 h after final challenge,mice were performed to examine airway responsiveness;bronchoalveolar lavage fluid(BALF)was harvested for cell counting and statistical classification of inflammatory cells through flow cytometry analysis.Pulmonary slides were collected for pathological examination,including HE,PAS,Masson and α-SMA immunohistochemical staining.ELISA was used to detect levels of IFN-γ,IL-4,IL-5,IL-13,IL-17A in lung tissue and serum,as well as serum total IgE and MPE-specific IgE,IgG1,IgG2a levels.Results:Pathological examination showed higher airway reactivity,more inflammatory cells infiltration around airway,obvious goblet metaplasia,thickening of airway smooth muscles and dramatical fibrin deposition around airway in model group.Total cell numbers of BALF were increased from<1×105 cells/ml in P-P group to>5×105 cells/ml in model group,in which eosinophils were predominant cellular type,levels of IL-4,IL-13,IL-17A in lung and IL-5,IL-13 levels in serum were significantly increased,as well as significant increasing levels of total IgE and MPE-specific IgE,IgG1,IgG2a.Conclusion:MPE-sensitized and challenged mice induces typical eosinophilic asthma featured with elevated eosinophils,as well as secretion of inflammatory factors of type 2 and type 17,IgE,IgG1 and IgG2a subtypes soars to high levels.关键词
艾蒿/过敏性哮喘/嗜酸性粒细胞/IgEKey words
Mugwort/Allergic asthma/Eosinophils/IgE分类
医药卫生引用本文复制引用
周玲慧,李林梅,谢焕城,宋仕杰,何颖,陶爱林..艾蒿花粉诱导小鼠过敏性哮喘及其内在型研究[J].中国免疫学杂志,2024,40(1):52-57,6.基金项目
国家自然科学基金(81871266). （81871266）
