中国免疫学杂志2024，Vol.40Issue(1)：58-66,9.DOI:10.3969/j.issn.1000-484X.2024.01.007
骨肉瘤中铜死亡相关亚型鉴定、预后模型构建以及免疫细胞浸润分析
Identification of copper death related subtypes,construction of prognosis model and analysis of immune cell infiltration in osteosarcoma
摘要
Abstract
Objective:To analyze identification of copper death gene related subtypes,construction of prognosis model and influence of immune infiltration in osteosarcoma(OS)on basis of copper death gene.Methods:Survival and prognosis of OS associated copper death gene were analyzed combining by TARGET and GEO database.OS was divided into different subtypes of copper death by consistent clustering method.SSGSEA was used to analyze difference of immune cells in classification of copper death.Setting P value= 0.05 and q value=0.05,GO and KEGG enrichment analysis were performed on differential genes of copper death typing.Prognosis model was constructed according to results of Lasso regression analysis and cross validation,risk assessment analysis and ROC curve were used to evaluate accuracy of model prediction.Combined with clinical characteristics,nomograms were constructed to predict survival time of patients,and risk differences were analyzed.Immune cell infiltration and tumor microenvironment analysis were performed on OS samples."pRRophetic"package in R software was used to analyze drug sensitivity of OS samples.Results:FDX1,GLS,DLAT and PDHB as high-risk genes for OS prognosis were identified.According to copper death classification of OS samples,OS could be divided into two types:CRGclusterA and CRGclusterB.CRGclusterA was associated with Th2 cells,and CRGclusterB was associated with Th1 cells.Most OS copper death genes were highly expressed in CRGclusterA.Immune cell infiltration analysis results showed that γδ T cells,resting mast cells and resting dendritic cells were positively correlated with risk score,while CD8 T cells were negatively correlated with risk score.Drug sensitivity analysis showed that OS showed higher sensitivity to Elesclomol and GW.441756.Conclusion:Two subtypes of CRGclusterA and CRGclusterB are identified in this study.Four high-risk prognostic genes FDX1,GLS,DLAT and PDHB are identified,providing new insights into prognostic evaluation and immunotherapy target candidates for OS.关键词
铜诱导细胞死亡/骨肉瘤/一致性聚类/免疫浸润/预后模型Key words
Copper-induced cell death/Osteosarcoma/Consistent clusters/Immune infiltration/Prognostic model分类
医药卫生引用本文复制引用
邵子晨,李华南,章晓云,孙伟康,袁启鹏..骨肉瘤中铜死亡相关亚型鉴定、预后模型构建以及免疫细胞浸润分析[J].中国免疫学杂志,2024,40(1):58-66,9.基金项目
国家自然科学基金项目(82060871,81860857) （82060871,81860857）
江西省自然科学基金项目(20202BAB206071). （20202BAB206071）
