中国免疫学杂志2024，Vol.40Issue(1)：78-81,4.DOI:10.3969/j.issn.1000-484X.2024.01.010
HPV L1保守序列多肽抗血清降解HPV6感染能力的测试
Test of the ability of HPV L1 conserved sequence polypeptide to antisera to degrade HPV6 infection
摘要
Abstract
Objective:To determine whether human papillomavirus(HPV L1)C-terminal conserved sequence antibodies with cross-reactive major capsid proteins of different types of HPV L1 have the ability to degrade HPV6 infection.Methods:Condyloma specimens were collected,HPV6 infection cases were identified from the collected samples,and virus was extracted.Polypeptide anti-sera were diluted in different proportions,and then co-cultured and neutralized with the resulting virus,then removed to contact mono-layer-cultured human immortalized keratinocytes and tested by HPV6 disease using PCR.Content of HPV6 DNA in human immortalized keratinocytes was exposed,and the presence of HPV6 L1 protein in this cells was tested by ELISA.Results:Human immortalized ke-ratinocytes infected with HPV6 virus neutralization at different dilution concentrations,the PCR products of their DNA extracts were electrophoresis and showed positive bands of HPV6 specificity zone at 280 bp of the gel,and the intensity of positive bands gradually decreased with increasing antiserum concentration.Protein extracted from human immortalized keratinocytes exposed to anti-serum neutralizing virus was tested by ELISA,and the amount of HPV L1 protein showed the same gradient trend as the above PCR test results,and the difference were statistically significant.Conclusion:It is preliminarily proved that HPV6 L1 conserved sequence polypeptide antisera can partially degrade the infection ability of the virus,and it has the value of studying more HPV neutralization types.关键词
HPV/多肽抗血清/人永生化角质形成细胞/降解/体外感染Key words
HPV/Polypeptide antiserum/Human immortalized keratinocytes/Degradation/In vitro infection分类
医药卫生引用本文复制引用
邓金芳,李志英,薛冰,肖长义..HPV L1保守序列多肽抗血清降解HPV6感染能力的测试[J].中国免疫学杂志,2024,40(1):78-81,4.基金项目
国家卫生健康委医药卫生科技发展研究中心项目(HDSL202004006) （HDSL202004006）
湖北省卫生健康委员会联合基金项目(WJ2019H535). （WJ2019H535）
