中国免疫学杂志2024，Vol.40Issue(1)：82-85,4.DOI:10.3969/j.issn.1000-484X.2024.01.011
miR-146a调控TLR4/NF-κB通路对脑出血模型大鼠的保护作用及机制研究
Protective effect and mechanism of TLR4/NF-κB pathway regulated by miR-146a in intracerebral hemorrhage model rats
摘要
Abstract
Objective:To explore role of miR-146a in regulating TLR4/NF-κB pathway on inflammatory injury and neuropro-tection in intracerebral hemorrhage model rats and its possible mechanism.Methods:A total of 40 rats were selected and randomly divided into sham,model,over-expressing miR-146a adenovirus and negative virus injection groups,with 10 rats in each group.Garcia score was used for neurological function;HE staining was used to observe changes of brain tissues.ELISA was used to detect inflammatory factors levels.TLR4,NF-κB protein and gene expressions in brain tissues were detected by Western blot and RT-PCR.Results:Compared with model group,neural function score of overexpressed miR-146a adenovirus injection group was increased(P<0.05).Model group had abnormal cell morphology,edema and inflammation.Cell morphology,edema and inflammation were alleviated in overexpressed miR-146a adenovirus injection group.Inflammatory factors levels in model group were higher than sham group(P<0.05).Inflammatory factors levels in overexpressed miR-146a adenovirus injection group were lower than model group(P<0.05).TLR4,NF-κB protein and mRNA expressions in model group were increased than sham group(P<0.05).TLR4,NF-κB protein and mRNA expressions in overexpressed miR-146a adenovirus injection group were decreased than model group(P<0.05).Conclusion:miR-146a can improve neural function and reduce inflammatory injury in rats with intracerebral hemorrhage,possibly by inhibiting activation of TLR4/NF-κB signaling pathway and reducing inflammatory factors levels of brain tissues.关键词
miR-146a/TLR4/NF-κB通路/脑出血/保护作用Key words
miR-146a/TLR4/NF-κB pathway/Intracerebral hemorrhage/Protective effect分类
医药卫生引用本文复制引用
吴俊波,杨杰,肖锋,李金亮..miR-146a调控TLR4/NF-κB通路对脑出血模型大鼠的保护作用及机制研究[J].中国免疫学杂志,2024,40(1):82-85,4.基金项目
江西省卫生健康委科技计划项目(202212091). （202212091）
