中国免疫学杂志2024，Vol.40Issue(1)：92-96,5.DOI:10.3969/j.issn.1000-484X.2024.01.013
基于IL-17/NF-κB信号通路探讨敦煌医方大补脾汤联合奥沙利铂对胃癌荷瘤小鼠炎症免疫的影响
Effect of Dunhuang medical prescription Dabupi Decoction combined with oxaliplatin on inflammatory immunity of gastric cancer-bearing mice based on IL-17/NF-κB signaling pathway
摘要
Abstract
Objective:To study anti-tumor effect of Dunhuang medical prescription Dabupi Decoction on gastric cancer-bearing mice and effect of Dabupi Decoction combined with oxaliplatin on inflammatory immunity of gastric cancer-bearing mice based on IL-17/NF-κB signaling pathway.Methods:Mouse model of MFC gastric cancer subcutaneously bearing tumor was established and ran-domly divided into model group,oxaliplatin group,high,medium and low doses of Dabupi Decoction combined with oxaliplatin groups[21.58,10.79,5.40 g/(kg·d)],with 10 mice in each group,male and female cage rearing.Administration began after 8 days of inoculation and continued for 14 days;next day after the last administration,eyeball blood was taken,mice were killed,tumor tissues were taken and weighed,and tumor inhibition rate was calculated.ELISA was used to detect contents of IL-17 and IL-6 in mice serum,immunohistochemistry(IHC),RT-qPCR and Western blot were used to detect IL-17,IL-6,NF-κB and p-NF-κB mRNA and protein expressions in mice tumor tissues,respectively.Results:Tumor inhibition rates of oxaliplatin group,high,medium and low doses of Dabupi decoction combined with oxaliplatin groups were 33.02%,52.92%,46.33%and 39.52%,respectively,and tumor quality of each treatment group was significantly lower than that of model group(P<0.01).High,medium and low doses of Dabupi Decoction combined with oxaliplatin groups were higher than that of oxaliplatin group.Compared with model group,contents of IL-17 and IL-6 in serum and expressions of IL-17,IL-6,NF-κB p65 and pNF-κB p65 mRNA and protein in tumor tissues in each treatment group were significantly reduced(P<0.01,P<0.05).Compared with oxaliplatin group,levels of IL-17 and IL-6 in serum and expres-sions of IL-17,IL-6,NF-κB p65 and pNF-κB p65 mRNA and protein in tumor tissues in high and medium doses of Dabupi Decoction combined with oxaliplatin groups were significantly reduced(P<0.01,P<0.05).Conclusion:Dunhuang medical prescription Dabupi Decoction has a certain anti-tumor effect on MFC gastric cancer-bearing mice,which can regulate inflammatory immunity and inhibit occurrence and development of gastric cancer by inhibiting IL-17/NF-κB signaling pathway.关键词
大补脾汤/胃癌/IL-17/NF-κB信号通路/炎症免疫Key words
Dabupi Decoction/Gastric cancer/IL-17/NF-κB signaling pathway/Inflammatory immunity分类
医药卫生引用本文复制引用
曾元丁,苏韫,龚红霞,牛世伟,张晗..基于IL-17/NF-κB信号通路探讨敦煌医方大补脾汤联合奥沙利铂对胃癌荷瘤小鼠炎症免疫的影响[J].中国免疫学杂志,2024,40(1):92-96,5.基金项目
国家自然科学基金(81660744) （81660744）
敦煌医学与转化教育部重点实验室开放基金(DHYX19-08,DHYX14-006) （DHYX19-08,DHYX14-006）
甘肃省自然科学基金(20JR10RA322) （20JR10RA322）
甘肃中医药大学中西医结合基础学科科研培育项目(5). （5）
