中国免疫学杂志2024，Vol.40Issue(1)：97-102,109,7.DOI:10.3969/j.issn.1000-484X.2024.01.014
乌梅丸通过调控NLRP3炎症小体改善溃疡性结肠炎大鼠肠黏膜炎症反应及细胞焦亡
Wu-Mei-Wan improves colonic mucosal inflammation and pyroptosis in rats with ulcerative colitis via regulating NLRP3 inflammasome
摘要
Abstract
Objective:To study effect and mechanism of Wu-Mei-Wan on improving colonic mucosal inflammatory response and pyroptosis in rats with ulcerative colitis(UC)via regulating NOD-like receptor family pyrin domain containing 3(NLRP3)inflam-masome.Methods:Male SD rats were selected for animal experiments.UC model was established by enema with 2,4,6-trinitroben-zene sulfonic acid.After model was established,different doses of Wu-Mei-Wan were given by gavage,negative control(NC)-lentivi-rus(LV)or NLRP3-LV were injected by tail vein.Disease activity index(DAI)of rats in each group was evaluated,length of colon was measured,pathological changes and histopathological scores,contents of IL-1β,IL-18,GSDMD-N,NLRP3 and Cleaved cas-pase-1 expressions in colon tissues were detected.Results:Typical UC pathological changes were found in colon mucosa of UC group,DAI,histopathological score,IL-1β,IL-18 contents,GSDMD-N,NLRP3 and Cleaved caspase-1 expressions in colon tissues of UC group were higher than control group,and colon length was shorter than control group(P<0.05).UC pathological changes of colon mucosa of rats in different concentrations of Wu-Mei-Wan groups were improved,DAI,histopathological score,IL-1β,IL-18 con-tents,GSDMD-N,NLRP3 and Cleaved caspase-1 expressions in colon tissues were lower than UC group,and colon length was longer than UC group(P<0.05).LV was injected simultaneously with intragastric administration of high-dose Wu-Mei-Wan,pathological changes of UC in colon mucosa of rats in NLRP3-LV+NC+high-dose Wu-Mei-Wan group was aggravated,DAI,histopathological score,IL-1β,IL-18 contents,GSDMD-N,NLRP3 and Cleaved caspase-1 expressions in colon tissues were higher than NC-LV+NC+ high-dose Wu-Mei-Wan group,and colon length was shorter than NC-LV+NC+high-dose Wu-Mei-Wan group(P<0.05).Conclusion:Wu-Mei-Wan improves colonic mucosal inflammation and pyroptosis in UC rats by inhibiting NLRP3 inflammasome.关键词
溃疡性结肠炎/乌梅丸/NLRP3炎症小体/炎症反应/细胞焦亡Key words
Ulcerative colitis/Wu-Mei-Wan/NLRP3 inflammasome/Inflammatory response/Pyroptosis分类
医药卫生引用本文复制引用
李克亚,王真权,王军文..乌梅丸通过调控NLRP3炎症小体改善溃疡性结肠炎大鼠肠黏膜炎症反应及细胞焦亡[J].中国免疫学杂志,2024,40(1):97-102,109,7.基金项目
2022年度湖南省中医药科研计划项目(D2022035) （D2022035）
2022年度湖南省自然科学基金青年基金项目(2022JJ40325). （2022JJ40325）
