中国免疫学杂志2024，Vol.40Issue(1)：110-115,6.DOI:10.3969/j.issn.1000-484X.2024.01.016
基于JAK2/STAT3信号通路探究利咽糖浆对慢性咽炎大鼠咽喉组织损伤及咽黏膜修复的影响
Influences of Liyan syrup on pharyngeal tissue injury and pharyngeal mucosal repair in rats with chronic pharyngitis based on JAK2/STAT3 signaling pathway
摘要
Abstract
Objective:Based on the Janus kinase 2(JAK2)/signal transducer and activator of transcription 3(STAT3)signaling pathway,to explore the influences of Liyan syrup on pharyngeal tissue injury and pharyngeal mucosal repair in rats with chronic phar-yngitis.Methods:SD rats were randomly separated into control group,model group,Liyan syrup group,RO8191(JAK2/STAT3 acti-vator)group,Liyan syrup+RO8191 group,the model group and drug intervention group were treated with ammonia water to stimulate the pharynx to build a chronic pharyngitis model,while rats in control group were injected with an equal dose of normal saline into the pharynx.After the intervention of Liyan Syrup and RO8191,the general condition of rats and the apparent state of pharynx were detected,and the apparent state of pharynx was scored;the pathological changes of pharynx of rats were measured by HE staining;the proportions of CD4+T and CD8+T and the ratio of CD4+T/CD8+T in peripheral blood T lymphocyte subsets of rats were measured by flow cytometry;the levels of serum tumor necrosis factor alpha(TNF-α),IL-6,IL-10,malondialdehyde(MDA),reactive oxygen species(ROS)and total antioxidant capacity(T-AOC)were measured by kits;the expressions of JAK2/STAT3 pathway-related proteins in rat pharyngeal tissues were measured by immunoblotting.Results:Compared with control group,the pharyngeal tissue of model group showed obvious pathological morphological damage,the peripheral blood CD4+T proportion and CD4+T/CD8+T,IL-10 and serum T-AOC level decreased(P<0.05),the pharyngeal appearance state score,CD8+T ratio,serum TNF-α,IL-6,MDA and ROS levels,and pharyngeal tissue p-JAK2/JAK2 and p-STAT3/STAT3 levels increased(P<0.05);compared with model group and Liyan syrup+ RO8191 group,the pathological and morphological damage of the pharynx of rats in Liyan syrup group was alleviated,the peripheral blood CD4+T and CD4+T/CD8+T,IL-10,and serum T-AOC level all increased(P<0.05),and the pharyngeal appearance state score,CD8+T,serum TNF-α,IL-6,MDA and ROS levels,and pharyngeal tissue p-JAK2/JAK2 and p-STAT3/STAT3 levels all decreased(P<0.05);the change trend of each index in RO8191 group was opposite to that in Liyan syrup group.Conclusion:Liyan syrup can reduce inflammation and oxidative stress by inhibiting the activation of JAK2/STAT3 signal,enhance anti-inflammatory and antioxidant capacity,relieve throat tissue damage and repair pharyngeal mucosa in rats with chronic pharyngitis.关键词
Janus激酶2/信号转导和转录激活因子3/利咽糖浆/慢性咽炎/咽喉组织/损伤/咽黏膜修复Key words
Janus kinase 2/signal transducer and activator of transcription 3/Liyan syrup/Chronic pharyngitis/Throat tissue/Injury/Pharyngeal mucosa repair分类
医药卫生引用本文复制引用
李勇,沈文明,王文茜..基于JAK2/STAT3信号通路探究利咽糖浆对慢性咽炎大鼠咽喉组织损伤及咽黏膜修复的影响[J].中国免疫学杂志,2024,40(1):110-115,6.基金项目
武汉市医学科研项目(WZ20D12). （WZ20D12）
