中国免疫学杂志2024，Vol.40Issue(1)：116-121,6.DOI:10.3969/j.issn.1000-484X.2024.01.017
长链非编码RNA ZIM2-AS1在肝细胞癌中的表达及其临床意义
Expression and clinical significance of long non-coding RNA ZIM2-AS1 in hepatocellular carcinoma
摘要
Abstract
Objective:To explore the expression of long non-coding RNA(lncRNA)ZIM2-AS1 in hepatocellular carcinoma(HCC)and its clinical significance as well as diagnostic value using the data obtained from the Cancer Genome Atlas(TCGA).Meth-ods:The transcriptome sequencing(RNA-seq)data and clinical information of 374 HCC tissues and 50 paired paracancerous tissues were gathered from the TCGA database,then the expression trends of ZIM2-AS1 in HCC and its correlation with clinicopathological features,prognosis,immune cell infiltration,as well as diagnostic value was inspected by bioinformatics analysis using relevant R packages.The expression of ZIM2-AS1 in human normal liver cell line and HCC cell lines was examined by qRT-PCR.Results:The ex-pression of ZIM2-AS1 was highly expressed in HCC tissues(P<0.001),and its expression level was significantly correlated with age,gender,N stage,histologic grade and AFP level(P all<0.05).The overall survival(OS)and disease specific survival(DSS)of patients with high ZIM2-AS1 expression were significantly shorter than those of patients with low expression(P<0.05),and ZIM2-AS1 was an in-dependent risk factor affecting OS.Immune cell infiltration analysis showed that ZIM2-AS1 was closely related to the infiltration of Th2 cells,CD56brightNK cells,follicular helper T cells(Tfh),neutrophils and plasmacytoid dendritic cells(pDC)(|Spearman's r|>0.1,P<0.05)in HCC.ROC curve analysis revealed that the expression level of ZIM2-AS1 possesse potential diagnostic value in HCC,N0 stage,histologic grade G1 and G2,OS and DSS(AUC all>0.50).qRT-PCR results showed that the expression level of ZIM2-AS1 in HCC cell lines was significantly higher than that in human normal liver cells(P all<0.05).Conclusion:The elevated expression of lncRNA ZIM2-AS1 is an independent risk factor for poor prognosis of HCC patient and has potential application value as a biomarker for HCC diagnosis,prognosis as well as tumor immune microenvironment assessment.关键词
长链非编码RNA/ZIM2-AS1/肝细胞癌/预后/诊断/免疫细胞浸润Key words
Long non-coding RNA/ZIM2-AS1/Hepatocellular carcinoma/Prognosis/Diagnosis/Immune cell infiltration分类
临床医学引用本文复制引用
孙晋,李英楠,石梦姣,田红卫,慕艳华,李君,李宗芳..长链非编码RNA ZIM2-AS1在肝细胞癌中的表达及其临床意义[J].中国免疫学杂志,2024,40(1):116-121,6.基金项目
国家自然科学基金(81502136) （81502136）
陕西省自然科学基础研究计划项目(2023-JC-YB-750). （2023-JC-YB-750）
