中国免疫学杂志2024，Vol.40Issue(1)：156-162,中插8-中插9,9.DOI:10.3969/j.issn.1000-484X.2024.01.024
miR17-92簇在痛风中的表达及临床意义
Expression and clinical significance of miR17-92 cluster in gout
摘要
Abstract
Objective:To explore expression of each member of miR17-92 cluster in peripheral blood mononuclear cells(PBMCs)of patients with gout,to predict their possible targets and pathways of action,and to evaluate their possible mechanism and clinical significance in gout.Methods:A total 67 gouty arthritis(GA)patients were selected,including 22 patients with acute gout arthritis(AG)and 45 patients with intermittent gout(IG),and 35 normal health control(HC)were selected in Affiliated Hospital of North Sichuan Medical College.RT-qPCR measured expressions of miR17-92 cluster,IFN-γ,IL-10 and some members of JAK-STAT pathway,and relevant laboratory indicators were collected to analyze correlation between each other.Results:Relative expressions of miR17,miR18a,miR19a,miR20a and miR19b were significantly changed in AG,IG and HC(H=8.753,P<0.05;H=6.338,P<0.05;H=6.523,P<0.05;H=9.061,P<0.05;H=9.729,P<0.01).JAK3 and STAT2 expressions were statistically different in AG,IG and HC groups(H=10.349,P<0.01;H=14.801,P<0.01).Expression of IFN-γ was statistically different among AG,IG and HC groups(H=8.734,P<0.05).In AG patients,miR18a expression was inversely correlated with IBIL,Crea,MO and HGB.miR19a ex-pression was negatively associated and TC,UA and HGB.miR20a expression was negatively associated with Crea.miR19b expression was negatively associated with UA and HGB.In IG patients,miR17 expression was negatively associated with IBIL,WBC,LY and MO.miR18a expression was positively associated with ALP,miR19a expression was negatively associated with TC and UA,and miR20a expression was negatively associated with ADA and UA.Conclusion:miR17-92 cluster may regulate development and partici-pate in clinical pathology of gout by targeting JAK-STAT pathway.关键词
痛风/miR17-92簇/JAK-STAT通路/JAK3/STAT2/IFN-γKey words
Gout/miR17-92 cluster/JAK-STAT pathway/JAK3/STAT2/IFN-γ分类
医药卫生引用本文复制引用
冯丹,姜蓉琼,杨桂钊,张惠,王丹,刘静,余成秀,袁国华..miR17-92簇在痛风中的表达及临床意义[J].中国免疫学杂志,2024,40(1):156-162,中插8-中插9,9.基金项目
湖北省重点实验项目(OIR13004A). （OIR13004A）
