中国人兽共患病学报2023，Vol.39Issue(12)：1139-1145,7.DOI:10.3969/j.issn.1002-2694.2023.00.147
狂犬病病毒基质蛋白74位氨基酸位点对其线粒体定位的影响
Effects of histidine 74 in rabies virus matrix protein on mitochondrial localization
摘要
Abstract
This study investigated the effect of histidine(H)at position 74 on the localization of full-length viral matrix protein(M)in mitochondria.A eukaryotic expression vector expressing the N-terminus or C-terminus of wild-type M protein or its histidine 74 mutant protein(H→P,H74P)fused with V5(tag protein)-APEX2(modified ascorbate peroxidase)or A-PEX2-V5 was constructed and transfected into 293T cells.Fluorescence staining and laser confocal microscopy were used to an-alyze the effect of histidine 74 mutation on the localization of full-length M protein in mitochondria.Immunoblotting showed that the eukaryotic expression vector expressed the fusion protein normally.Fluorescence staining indicated that the H→P mu-tation at position 74 did not affect the ability of the full-length viral M protein to localize to mitochondria.Our findings regard-ing the effect of histidine 74 on the mitochondrial localization of full-length virus M protein provide a foundation for future study of the biological function of M protein and the replication mechanism of rabies virus.关键词
狂犬病病毒/M蛋白/线粒体定位Key words
rabies virus/M protein/mitochondrial localization分类
医药卫生引用本文复制引用
张曦,王姝捷,周俊,高琛,刘琴,周宏婧,许运斌..狂犬病病毒基质蛋白74位氨基酸位点对其线粒体定位的影响[J].中国人兽共患病学报,2023,39(12):1139-1145,7.基金项目
国家自然科学基金项目(No.31860705) （No.31860705）
贵州省科技计划项目(No.黔科合基础[2020]1Y061)Supported by the National Natural Science Foundation of China(No.31860705)and the Guizhou Provincial Science and Tech-nology Project(No.QKHJC[2020]1Y061) （No.黔科合基础[2020]1Y061）
