中国水产科学2023，Vol.30Issue(8)：933-941,9.DOI:10.12264/JFSC2023-0145
翘嘴鳜MyomiRs时空表达特征及靶向Pax7的预测分析
Spatio-temporal expression characteristics of MyomiRs and prediction analysis of MyomiRs targeting to Pax7 in Siniperca chuatsi
摘要
Abstract
MyomiRs,a class of muscle-specific microRNAs(miRNAs),play an important role in the proliferation and differentiation of myoblasts.This study investigated the spatio-temporal expression characteristics of four myomiRs(miR-1a,miR-133a-3p,miR-206,and miR-499)in Chinese perch(Siniperca chuatsi),as well as their expression features under short-term starvation stress,and predicted their regulation of Pax7.The expressions of the four myomiRs in various tissues of Chinese perch,induding white muscle at different stages of post embryonic development and white muscle after 5 days of starvation,were examined by real-time fluorescence quantitative PCR.Then,the targeting sites of the four myomiRs on the 3'-untranslated region(UTR)of Pax7 mRNA were predicted using RNAhybrid.The results showed that miR-1a,miR-133a-3p,and miR-206 were highly expressed at D60(60 days post hatching),while miR-499 was highly expressed at D100.Furthermore,miR-1a and miR-133a-3p were highly expressed in red muscle,white muscle,and the heart,yet low expressed in other tissues.miR-206 was highly expressed in red muscle and white muscle,while miR-499 was highly expressed in heart,followed by red muscle and white muscle,and was low expressed in other tissues without a significant difference observed.The expressions of all four myomiRs increased significantly after 5 days of starvation,suggesting that skeletal muscle may respond to starvation stress by upregulating the expression of the four myomiRs to regulate physiological processes such as metabolism.Target gene prediction analysis showed that miR-1a,miR-133a-3p,and miR-206 had binding sites on the 3'-UTR of Pax7.Further verification by real-time fluorescence quantitative PCR showed that the expression of miR-206 and Pax7 at various post embryonic developmental stages and the circadian rhythms of the Chinese perch were in opposite trends.These results suggest that miR-1a and miR-133a-3p are muscle-specific,while miR-206 and miR-499 are enriched in muscle-derived tissues.In addition,miR-206 may directly regulate the expression of Pax7,thus affecting the differentiation and development of muscle tissue in juvenile Chinese perch.关键词
翘嘴鳜/MyomiRs/骨骼肌/表达特征/昼夜节律Key words
Siniperca chuatsi/MyomiRs/skeletal muscle/expression features/circadian rhythms分类
农业科技引用本文复制引用
曾维,朱鑫,程聪益,孟杨杨,张宇,宾琴,李灿,褚武英,陈韬..翘嘴鳜MyomiRs时空表达特征及靶向Pax7的预测分析[J].中国水产科学,2023,30(8):933-941,9.基金项目
国家自然科学基金项目(U21A20263,31820103016,32002370) （U21A20263,31820103016,32002370）
湖南省自然科学基金项目(2021JJ40629). （2021JJ40629）
