中国水产科学2023，Vol.30Issue(8)：953-964,12.DOI:10.12264/JFSC2023-0106
紫贻贝产卵前后转录组分析与相关基因的筛选
Transcriptome analysis and screening of related genes before and after spawning of Mytilus galloprovincialis
摘要
Abstract
The aim of this study was to identify functional genes associated with the oviposition of Mytilus galloprovincialis and to elucidate the underlying mechanisms of oviposition.Transcriptomic analysis of female Mytilus galloprovincialis gonads was conducted before and after spawning.Pairwise comparisons of sequencing libraries from tissue samples were also performed.Before and after spawning,there were 4060 differential genes,with 1488 up-regulated and 2572 down-regulated genes.The GO functional enrichment terms are grouped into three functional categories:biological process,cellular component,and molecular function.Some genes were annotated by GO terms such as growth,developmental process,reproduction,and reproductive process.KEGG enrichment analysis revealed signaling pathways associated with oviposition and reproduction,including the TCA cycle,GnRH signaling pathway,ovarian steroidogenesis,estrogen signaling pathway,oxytocin signaling pathway,and steroid biosynthesis.In conjunction with GO and KEGG enrichment results,we identified five potential genes associated with reproduction:gonadotropin-releasing hormone receptor(GnRHR),luteinizing hormone β(LHβ),matrix metalloproteinase 14(MMP14),cytochrome P4503A enzyme(CYP3A),and cytochrome P45017A enzyme(CYP17A).The expression levels of LHβ and MMP14 genes increased,whereas the expression levels of GnRHR,CYP3A,and CYP17A genes decreased.These findings were validated using real-time quantitative PCR(RT-qPCR).The differential expression of the five genes mentioned above is crucial in regulating the synthesis of sex hormones and reproductive process during the spawning of Mytilus galloprovincialis,which is consistent with the results of transcriptome sequencing.In this study,we utilized transcriptome sequencing technology to analyze the differences in the gonad transcriptome of female Mytilus galloprovincialis before and after spawning.The aim was to identify the key pathways and candidate genes involved in spawning,which could provide valuable data for the breeding of Mytilus galloprovincialis.关键词
紫贻贝/转录组测序/性腺/产卵/差异表达基因Key words
Mytilus galloprovincialis/transcriptome sequencing/spawning/gonad/differentially expressed genes分类
农业科技引用本文复制引用
姚荣辉,叶莹莹,马颜雯,董震宇,郭锋,王伟峰,郭宝英..紫贻贝产卵前后转录组分析与相关基因的筛选[J].中国水产科学,2023,30(8):953-964,12.基金项目
国家自然科学基金面上项目(42076119) （42076119）
浙江省"三农九方"科技协作计划"揭榜挂帅"项目(2023SNJF065). （2023SNJF065）
