中国水产科学2023，Vol.30Issue(8)：989-999,11.DOI:10.12264/JFSC2023-0107
两种乳酸菌对尼罗罗非鱼生长及免疫性能的影响
Effects of two lactic acid bacteria on the growth and immune performance of Nile tilapia(Oreochromis niloticus)
摘要
Abstract
This study aimed to investigate the effects of feeding diets supplemented with Lactobacillus plantarum LP-37 and Pediococcus pentosaceus PP-23 on the growth and immune performance of Nile tilapia.Seven experimental groups were used in this study,namely,control group C0:feeding basic feed;L1-L3 groups:adding three different concentrations of Lactobacillus plantarum LP-37(1.0×106 CFU/g,1.0×107 CFU/g,1.0×108 CFU/g)to basic feed;P1-P3 groups:adding three different concentrations of Pediococcus pentosaceus PP-23(1.0× 106 CFU/g,1.0×107 CFU/g,1.0×108 CFU/g)to basic feed.The results showed that except for those in the L1 group,the final body weights of the fish in the experimental groups(123.22～158.60 g)were significantly higher than those of the C0 group fish(118.16±4.88)g(P＜0.05).Except for those in the L1 and Pl groups,the weight gain rates in the other experimental groups(128.69～156.20 g)were significantly higher than that in the C0 group(108.16±0.45)％(P＜0.05).The final body weight and weight gain rate of the fish in the L3[(150.00±6.66)g,(152.69±3.51)％]and P2[(153.24±5.36)g,(143.12±5.23)％]groups were significantly higher than those of the C0 group fish(P＜0.01).The addition of different concentrations of LP-37 and PP-23 was able to improve activities of three digestive enzymes of Nile tilapia.Among various groups,the greatest increases appeared in the L3 and P2 groups.In L3,protease(PRS)was(1.39±0.06)U/mg,α-amylase(AMS)was(55.20±3.39)U/dL,and alkline phosphatase(AKP)was(3.28±0.28)U/L.In P2,PRS was(1.40±0.04)U/mg,α-amylase(AMS)was(65.28±11.50)U/dL,and alkaline phosphatase(AKP)was(3.50±0.36)U/L.Compared with the expression in the C0 group,IL-1βand IFN-γ genes in the spleen of the fish in the experimental groups were significantly down-regulated(P＜0.05),and the highest down-regulation occurred in the L3 and P2 groups.IL-1β gene in the head kidney of fish of various experimental groups was also significantly down-regulated(P＜0.05),and the highest down-regulation occurred in the L3 and P2 groups.In terms of intestinal tissue morphology,the villous height in various experimental groups was significantly higher than that in the C0 group(P＜0.05).The crypt depth,the ratio of villous height to crypt depth,and muscle layer thickness in the L3 and P2 groups were significantly higher than those in the other experimental and control groups(P＜0.05),whereas there was no significant difference in these parameters between the other experimental and control groups(P＞0.05).In summary,adding 1.0×108CFU/g Lactobacillus plantarum LP-37 and(1.0×107)CFU/g Pediococcus pentosaceus PP-23 to the diet can significantly increase the growth rate,digestive enzyme activity,and intestinal tissue structure,and alter the expression of certain immune related genes in Nile tilapia.关键词
植物乳杆菌/戊糖片球菌/尼罗罗非鱼/生长性能/免疫性能Key words
Lactobacillus plantarum/Pediococcus pentosaceus/Nile tilapia/growth performance/immune performance分类
农业科技引用本文复制引用
路广金,陈红菊,张冲,周建平,赵亚选,李岩,姚智磊,于明,陈广韬,季相山..两种乳酸菌对尼罗罗非鱼生长及免疫性能的影响[J].中国水产科学,2023,30(8):989-999,11.基金项目
山东省重点研发计划项目(2021CXGC010806 （）
2021LZGC027) （）
山东省现代农业产业技术体系项目(SDAIT-13) （SDAIT-13）
山东省农业良种工程项目(2019LZGC013,2019LZGC020). （2019LZGC013,2019LZGC020）
