中国实验动物学报2023，Vol.31Issue(12)：1539-1544,6.DOI:10.3969/j.issn.1005-4847.2023.12.003
长春瑞滨诱导化疗性静脉炎大鼠模型的构建和评价
Construction and evaluation of a chemotherapeutic phlebitis rat model induced by vinorelbine via the dorsalis pedis vein
摘要
Abstract
Objective To establish and evaluate chemotherapeutic phlebitis model rats induced by vinorelbine via the dorsalis pedis vein.Methods Rats were divided randomly into control and 4 different concentration of vinorelbine-induced model groups.Control rats were injected with 0.1 mL normal saline via the dorsalis pedis vein of the hind limb,while other rats were injected with different concentrations of vinorelbine(2,3,4,5 mg/mL),as above.General observations were performed and the hind limb volume was measured daily for 7 consecutive days to calculate the swelling rate.The rats were then killed and histological changes in the dorsalis pedis vein were observed by hematoxylin and eosin staining.Microstructural changes on the surface of the vascular endometrium were observed by scanning electron microscopy.Results Injection of 2,3,4,5 mg/mL vinorelbine via the dorsalis pedis vein significantly induced hind limb swelling in a concentration-dependent manner,peaking on day 3 in each group.The phlebitis rates on day 7 were 50%in the 2 mg/mL group and 83.3%in the 3 mg/mL group.Phlebitis was also induced in the 4 mg/mL and 5 mg/mL groups,including grade Ⅲ in 66.6%and grade Ⅳ in 83.3%.Histopathology showed inflammatory cell infiltration,wall thickening,lumen stenosis,and thrombosis in the tissues surrounding the veins.Scanning electron microscopy showed destruction of tight junctions of venous endothelial cells,and a rough surface of the vascular lining,resultsing in blood cell adhesion.Conclusions Injection of 0.1 mL of 3～5 mg/mL vinorelbine via the dorsalis pedis vein could induce red,swollen,and cord-like veins,as well as infiltration of inflammatory cells around the vein,thickened vein walls,lumen stenosis,and thrombosis.In addition,the surface of the venous intima was rough and adhered to numerous blood cells.All these features are consistent with those of clinical chemotherapeutic phlebitis in terms of the symptoms and pathological structure.关键词
化疗性静脉炎/大鼠/足背静脉/长春瑞滨Key words
chemotherapeutic phlebitis/rats/dorsalis pedis vein/vinorelbine分类
生物科学引用本文复制引用
王淑敏,郝淑兰,冯玛莉,姜萌,王永辉,高振飞,王晞星,吉海杰..长春瑞滨诱导化疗性静脉炎大鼠模型的构建和评价[J].中国实验动物学报,2023,31(12):1539-1544,6.基金项目
国家自然科学基金面上项目(81973672),山西省科技创新人才团队专项资助(202204051001032),山西省基础研究计划(202203021221291),山西中医药大学科技创新团队(2022TD2005). Funded by the National Natural Science Foundation of China(81973672),the Special Fund for Science and Technology Innovation Team of Shanxi Province(202204051001032),Fundamental Research Program of Shanxi Province(202203021221291),Scientific and Technological Innovation Team of Shanxi University of Chinese Medicine(2022TD2005). （81973672）
