中国兽医科学2024，Vol.54Issue(1)：56-62,7.DOI:10.16656/j.issn.1673-4696.2024.0013
鸡圆圈病毒3型SYBR Green Ⅰ实时荧光定量PCR方法的建立与应用
Establishment and application of SYBR Green Ⅰ real-time fluorescence quantitative PCR method for chicken circle virus type 3
摘要
Abstract
In this study,a standard plasmid based on GyV3 VP2 gene was constructed and used as a template to establish a standard curve.On the basis of optimizing the qPCR reaction system,the specificity,sensitivity and repeatability of this method were evaluated.The results revealed the method was only specific for GyV3 amplification,the minimum detection template concentration was 1.585 × 101 copies/μL,and the coefficient of variation in both intra-class and intra-group repea-tability tests was less than 1.0％.The qPCR method established in this study was used to detect 50 clinical samples,and the detection rate was 10％(5/50),higher than the conventional PCR detection rate of 8％(4/50).The results above indicate that the real-time fluorescent quantitative PCR method established in this study has high specificity,reproducibility,and sensitivity and can be utilized for quick clinical detection of GyV3.关键词
圆圈病毒3型/VP2基因/实时荧光定量PCRKey words
gyrovirus 3/VP2 gene/real-time fluorescence quantitative PCR分类
农业科技引用本文复制引用
牛群,庄丽云,雷天宇,戴婷婷,孟庆福,刘荣昌,何华,郑新添..鸡圆圈病毒3型SYBR Green Ⅰ实时荧光定量PCR方法的建立与应用[J].中国兽医科学,2024,54(1):56-62,7.基金项目
国家现代农业产业技术体系资助项目(CARS-42) （CARS-42）
龙岩学院预防兽医学与生物技术福建省高等学校重点实验室开放基金项目(2022KF03) （2022KF03）
河南农业大学"拔尖人才"项目(30500737) （30500737）
