中国兽医科学2024，Vol.54Issue(1)：70-78,9.DOI:10.16656/j.issn.1673-4696.2024.0018
五株华南虎源弗氏柠檬酸杆菌的分离鉴定及其生物学特性研究
Isolation,identification and biological characteristics of five strains of Citrobacter fredii from South China tiger
摘要
Abstract
In order to understand the carrying status of potential pathogens of South China tiger in Meihuashan Breeding Institute of Fujian Province and analyze relevant biological characteristics,this study isolated bacteria from 104 tissues,feces,anus swab and nose swab of South China tiger collected from Meihuashan Breeding Institute of Fujian Province from 2018 to 2021.Biochemical identification,16S rRNA sequence amplification,virulence gene detection,pathogenicity test and drug susceptibility test were performed.The results showed that 5 strains of Citrobacter freundii were isolated from 104 South China tiger samples through isolation and culture,morphological observation,biochemical test and 16S rRNA sequence analysis.The sequence similarity of 16S rRNA among the five isolates was 99.3％-99.7％,and the similarity with the 18 strains of Citrobacter freundii from different sources was 98.3％-99.9％.Genetic analysis showed that the 5 isolates were closely related to the 18 strains of Citrobacter freundii in the same evolutionary branch.Virulence gene test results showed that FJ/Tiger-1803,FJ/Tiger-1804,FJ/Tiger-1806 and FJ/Tiger-1807 all carried genes ureD,ureE and ureABC;FJ/Tiger-1805 only carried genes ureD and ureE.The results of pathogenicity test in mice showed that healthy mice were infected by intrabitoneal injection at the same concentration(1 × 109 CFU/mL).The mortality rates of FJ/Tiger-1803,FJ/Tiger-1806,FJ/Tiger-1804,FJ/Tiger-1807 and FJ/Tiger-1805 in mice were 100％,100％,80％,80％and 0,respectively.The results of drug sensitivity test showed that the isolates had different resistance to 14 kinds of antibiotics,among which amoxicillin,penicillin G,vancomycin and erythromycin were the most resistant,and showed multiple resistance.In conclusion,five strains of Cit-robacter freundii with strong pathogenicity and severe drug resistance to mice were isolated from dif-ferent samples of South China tiger in this study,which should be paid attention to clinically.关键词
华南虎/弗氏柠檬酸杆菌/分离鉴定/毒力基因/耐药性Key words
Panthera tigris amoyensis/Citrobacter freundii/isolation and identification/viru-lence gene/drug resistance分类
农业科技引用本文复制引用
李雨琪,薛梦如,蓝佳萱,康娅莉,薛羽希,陈腾腾,林锡潘,林秋,谭聪灵,林开雄,范克伟,许静茹,朱智豪,蔡思思,周娴静,靳雨欣,刘璐,邓莹滋,陈芳婷..五株华南虎源弗氏柠檬酸杆菌的分离鉴定及其生物学特性研究[J].中国兽医科学,2024,54(1):70-78,9.基金项目
国家自然科学基金青年科学基金项目(31602048) （31602048）
福建省农业科技重大专项(2019NZ09005) （2019NZ09005）
福建省高校产学合作项目(2020N5012) （2020N5012）
福建省自然科学基金项目(2023J01990) （2023J01990）
国家级大学生创新创业训练计划项目(202011312012,202111312006,202311312009) （202011312012,202111312006,202311312009）
新罗区产学研科技联合创新项目(2022XLXYZ009) （2022XLXYZ009）
动物源性人兽共患病防控福建省高校工程研究中心开放基金项目(2022K008) （2022K008）
