中国兽医科学2024，Vol.54Issue(1)：134-140,7.DOI:10.16656/j.issn.1673-4696.2024.0020
绿原酸和咖啡酸对雷州山羊精液冷冻保存效果的影响
Effects of chlorogenic and caffeic acids on the cryopreservation of semen from Leizhou goats
摘要
Abstract
To improve the quality of frozen-thawed semen,this study investigated the effects of chlorogenic acid(CGA)and caffeic acid(CA)on the cryopreservation of semen from Leizhou goats.100 μmol/L CGA and CA were added to the freezing dilution solution,respectively.The group without addition served as the control,and cryopreservation experiment was conducted.The semen quality was evaluated by assessing frozen-thawed sperm viability,motility,survival rate,plasma membrane integrity,acrosome integrity,and antioxidant indicators such as reactive oxygen species(ROS)level,catalase(CAT),superoxide dismutase(SOD),glutathione peroxidase(GSH-PX),malondialdehyde(MDA),and mitochondrial membrane potential(MMP).The results showed that the addition of CGA and CA in the cryopreservation diluent significantly improved frozen-thawed semen quality(sperm motility,viability,survival rate,etc.)of Leizhou goats(P＜0.05).The enzyme activities of SOD,GSH-PX,CAT and MMP were ef-fectively increased(P＜0.05),and the sperm ROS level and MDA content were decreased(P＜0.05),so the cryopreser-vation effect of Lezhou goats semen was improved.The research results indicate that the quality and antioxidant properties of Leizhou goats semen are significantly improved after frozen-thawed by adding CGA and CA to the frozen preservation diluent,thereby enhancing the cryopreservation effect of Leizhou goats semen.关键词
绿原酸/咖啡酸/雷州山羊/精液冷冻保存/氧化应激Key words
chlorogenic acid/caffeic acid/Leizhou goats/cryopreservation of semen/oxidative stress分类
农业科技引用本文复制引用
苗丰帅,李靖,蒋欣怡,陈宣旭,于海滨,姜平,林紫薇,赵志辉,照那木拉..绿原酸和咖啡酸对雷州山羊精液冷冻保存效果的影响[J].中国兽医科学,2024,54(1):134-140,7.基金项目
广东省重点领域研发计划项目(2022B0202110002) （2022B0202110002）
广东省乡村振兴战略专项(2022-XPY-00-010) （2022-XPY-00-010）
广东省自然科学基金项目(2021A1515010867) （2021A1515010867）
粤西地区畜禽资源与种质创新重点实验室基金项目(2021A1515010867) （2021A1515010867）
广东海洋大学科研启动基金项目(R17032,060302052315) （R17032,060302052315）
