中国兽医杂志2023，Vol.59Issue(12)：26-33,8.
右美托咪啶对异氟烷诱导大鼠PC12细胞线粒体氧化损伤的影响
Effect of Dexmedetomidine on Isoflurane-induced Mitochondrial Oxidative Damage in Rat PC12 Cells
摘要
Abstract
In order to analyze the protective effect and mechanism of dexmedetomidine on isoflurane-induced mitochondrial oxida-tive damage in PC 12 cells,this study established control(Control)group,isoflurane(ISO)group,dexmedetomidine(DEX)group,and dexmedetomidine plus isoflurane(DEX+ISO)group.Reactive oxygen species(ROS),malondialdehyde(MDA),cata-lase(CAT),glutathione peroxidase(GPX),and superoxide dismutase(SOD)assay kits were used to detect cellular oxidative stress-related indicators.The mitochondrial membrane potential assay kit was used to measure mitochondrial membrane potential,the enzyme-linked immunosorbent assay(ELISA)kit was used to measure cytochrome C(Cyt-C)concentration,the terminal deoxynu-cleotidyl transferase-mediated dUTP nick end labeling(TUNEL)assay kit was used to detect cell apoptosis,and the Fura-2 AM fluo-rescent probe was used to measure intracellular calcium ion(Ca2+)concentration.The results showed that compared to the Control group,the ROS level and MDA content in PC 12 cells of the ISO group were significantly upregulated(P＜0.01),while the activi-ties of CAT,GPX,and SOD were significantly reduced(P＜0.01);compared to the ISO group,the ROS level in PC 12 cells of the DEX+ISO group was significantly reduced(P＜0.01),MDA content was significantly reduced(P＜0.05),and the activities of CAT and SOD were significantly increased(P＜0.01),while GPX activity was significantly increased(P＜0.05);compared to the Control group,the mitochondrial membrane potential in the ISO group significantly decreased(P＜0.01),while compared to the ISO group,the mitochondrial membrane potential in the DEX+ISO group significantly increased(P＜0.05);compared to the Control group,the Cyt-C concentration in the ISO group significantly increased(P＜0.01),and the cell apoptosis rate significantly increased(P＜0.01);compared to the ISO group,the Cyt-C concentration in the DEX+ISO group significantly decreased(P＜0.05),and the cell apoptosis rate significantly decreased(P＜0.01);compared to the Control group,the Ca2+concentration in the ISO group significantly increased(P＜0.01),while compared to the ISO group,the Ca2+concentration in the DEX+ISO group sig-nificantly decreased(P＜0.05).The results indicate that dexmedetomidine has a protective effect agsinst oxidative stress and mito-chondrial dysfunction induced by isoflurane in PC 12 cells.关键词
右美托咪啶/异氟烷/氧化应激/线粒体功能障碍Key words
dexmedetomidine/isoflurane/oxidative stress/mitochondrial dysfunction分类
畜牧业引用本文复制引用
关佳佳,刘萌萌,于志勇,于权麟,王思爽,李继文,迟良,刘焕奇..右美托咪啶对异氟烷诱导大鼠PC12细胞线粒体氧化损伤的影响[J].中国兽医杂志,2023,59(12):26-33,8.基金项目
山东省现代农业产业技术体系牛产业创新团队资助项目(SDAIT-09-05) （SDAIT-09-05）
