中国兽医杂志2023，Vol.59Issue(12)：34-40,7.
干酪乳杆菌SY13对小鼠免疫功能和肠道菌群的影响
Effects of Lactobacillus casei SY13 on Immune Function and Intestinal Flora of Mice
摘要
Abstract
Building on the previously validated lipid-lowering and probiotic functions of Lactobacillus casei SY13,combined with the characteristic of oligosaccharide promoting the colonization of probiotics in the intestines,this study aims to investigate the effects of SY 13 on the host's immune function and intestinal flora.Forty-eight six-week-old mice were divided into four groups:gavaged with sterile PBS(BP1 group),SY 13(BP2 group),SY13+fructo-oligosaccharide(BP3 group),and SY 13+lactulose(BP4 group)for 28 days.On the 1st,3rd,5th,and 7th days,blood samples were collected from three mice in each group for ELISA to determine serum secretory immunoglobulin A(sIgA)and cytokine levels(IL-1β,IL-2,IL-4 and IL-10).On the 1st day,TaqMan-MGB probes were used for real-time quantitative PCR to detect SY13 content in the intestinal contents of mice in each group.On the 1st and 7th days,the microbial diversity in the cecum of mice in each group was analyzed using Illumina Miseq.The results showed that on the 7th day,the serum sIgA level in the BP1 group was significantly lower than in the other groups(P＜0.05).On the 3rd day,the serum IL-1 β level in the BP4 group,and on the 5th day,the serum IL-4 and IL-10 levels in the BP4 group were significantly higher than in the other groups(P＜0.05).On the 1st day,the SY13 content in the jejunum,ileum,and cecum of the BP4 group was significantly higher than in the other groups(P＜0.05).On the 1st and 7th days,the abundance of Bacteroidetes in the cecum of the BP3 and BP4 groups was significantly higher than in the BP1 group(P＜0.05),and the ratio of Firmicutes to Bacteroidetes in the cecum of the BP3 group was lower than in the other groups.On the 7th day,the abundance of Alistipes in the cecum of the BP3 and BP4 groups was significantly higher than in the BP1 group(P＜0.05).The results indicate that SY13 promotes overall health,and concomitant use of SY 13 and lactulose regulates host intestinal mucosal immunity,improves intestinal flora,reduces the risk of intestinal infections,while simultaneous use of SY13 and fructo-oligosaccharide mainly exerts probiotic effects through interaction with intestinal flora.关键词
干酪乳杆菌SY13/小鼠/免疫功能/肠道菌群Key words
Lactobacillus casei SY 13/mice/immune function/intestinal flora分类
农业科技引用本文复制引用
贾震虎,杨云琪,王腾宇,逄晓阳,吕加平..干酪乳杆菌SY13对小鼠免疫功能和肠道菌群的影响[J].中国兽医杂志,2023,59(12):34-40,7.基金项目
国家重点研发计划项目(SQ2018YFC200044) （SQ2018YFC200044）
