中国兽医杂志2023，Vol.59Issue(12)：71-76,6.
替米考星对胞内金黄色葡萄球菌的抗菌活性
Antibacterial Activity of Tilmicosin Against Intracellular Staphylococcus aureus
摘要
Abstract
To explore the antibacterial activity of macrolides tilmicosin against intracellular Staphylococcus aureus(S.aureus),this study used S.aureus ATCC 29213 as the target bacterium and mouse monocyte macrophage cells(RAW264.7)as the host cells to construct an intracellular infection system of S.aureus.The growth curve of intracellular S.aureus was plotted,and the minimum inhibitory concentration(MIC),minimum bactericidal concentration(MBC),mutant prevention concentration(MPC),mutant selection window(MSW),post-antibiotic effect(PAE),and bactericidal curve of tilmicosin against intracellular S.aureus were determined.The results showed that intracellular S.aureus was in the lag phase,logarithmic phase,stationary phase,and decline phase at 0-4,4-18,18-24,and 24-48 hours,respectively.The MIC,MBC,MPC,and MSW were 4,8,12.8,and 4-12.8 μg/mL,respectively.The PAE and bactericidal curve results showed that tilmicosin exhibited a significant time-dependent killing characteristic against intracellular S.aureus.The results indicate that tilmicosin has strong antibacterial activity against intracellular S.aureus,providing a reference for developing rational dosing regimens for the treatment of bovine mastitis caused by intracellular S.aureus.关键词
替米考星/奶牛乳房炎/胞内金黄色葡萄球菌/抗菌活性/RAW264.7细胞Key words
tilmicosin/bovine mastitis/intracellular Staphylococcus aureus/antibacterial activity/RAW264.7 cells分类
农业科技引用本文复制引用
鞠牧洁,刘金环,蒋永涛,冷楠楠,罗万和..替米考星对胞内金黄色葡萄球菌的抗菌活性[J].中国兽医杂志,2023,59(12):71-76,6.基金项目
塔里木畜牧科技兵团重点实验室开放课题资助项目(HS202108) （HS202108）
塔里木大学校长基金资助项目(TDZKSS202144) （TDZKSS202144）
