中国兽医杂志2023，Vol.59Issue(12)：77-82,6.
鸡蛋中阿莫西林残留HPLC-DAD检测方法的建立
Establishment of HPLC-DAD Detection Method for Residual Amoxicillin in Eggs
肖裕章 1姚静 2孙毅 3毕海林 1李颜 1曾孝丽 1唐建华4
作者信息
- 1. 重庆方通动物药业有限公司,重庆荣昌 402460
- 2. 西南大学动物医学院,重庆荣昌 402460
- 3. 山东省海阳市综合行政执法大队(农业),山东海阳 265100
- 4. 重庆方通动物药业有限公司,重庆荣昌 402460||西南大学动物医学院,重庆荣昌 402460
- 折叠
摘要
Abstract
In order to accurate determine the residual amoxicillin in eggs,this study established a high-performance liquid chromatography-diode array detection(HPLC-DAD)method for detecting amoxicillin residues in eggs.Amoxicillin in egg liquid was extracted by acetonitrile precipitation of proteins,and pure water-saturated dichloromethane was used as solvent for further extraction and purification.The chromatographic conditions for the detection of amoxicillin residues in eggs were as follows:0.01 mol/L potassium dihydrogen phosphate solution(pH=5.5)and acetonitrile as the mobile phase,flow rate of 1.0 mL/min,detection wavelength at 230 nm,sample injection volume of 20 μL,and column temperature of 30 ℃.The results showed that the mass concentration of amoxicillin ranged from 0.05 to 100.00 μg/mL,exhibiting a good linear relationship with peak area(R2=0.999 8).The method demonstrated excellent repeatability,precision,and stability,with relative standard deviations within 2.0％.The average sample recovery rates ranged from 82.89％to 89.92％,with relative standard deviations ranging from 1.68％to 2.71％.The detection limit for amoxicillin was 0.025 μg/mL,and the quantification limit was 0.050 μg/mL.The results indicate that the HPLC-DAD method established in this study is simple,stable,and highly sensitive,making it suitable for the accurate detection of amoxicillin residues in eggs.关键词
高效液相色谱(HPLC)/二级管阵列检测器(DAD)/鸡蛋/阿莫西林/残留Key words
high-performance liquid chromatography(HPLC)/diode array detection(DAD)/egg/amoxicillin/residue分类
农业科技引用本文复制引用
肖裕章,姚静,孙毅,毕海林,李颜,曾孝丽,唐建华..鸡蛋中阿莫西林残留HPLC-DAD检测方法的建立[J].中国兽医杂志,2023,59(12):77-82,6.
