中国兽医杂志2023，Vol.59Issue(12)：129-134,6.
车前草多糖对高脂日粮饲喂小鼠脂代谢和氧化应激的影响
Effects of Plantain Polysaccharide on Lipid Metabolism and Oxidative Stress in Mice Fed with High-Fat Diet
摘要
Abstract
To investigate the impact of plantain polysaccharide(PLP)on lipid metabolism abnormalities and oxidative stress in mice fed with high-fat diet(HF),150 SPF male healthy Kunming mice were selected.After a week of adaptation,they were randomly divided into five groups:Control(CK)group,High-fat(HF)group,HF+100 mg/(kg·bw)PLP group,HF+200 mg/(kg·bw)PLP group,and HF+400 mg/(kg·bw)PLP group.Daily food and water intake were recorded,and after 4 weeks,liver and blood samples were collected for lipid metabolism and oxidative stress-related indicators and gene expression analysis.The results showed that compared with the CK group,mice in the HF group exhibited significantly elevated levels of serum triglycerides(TG),total cholesterol(TC),low-density lipoprotein cholesterol(LDL-C),and glucose(GLU),as well as increased aspartate transaminase(AST)and alanine transaminase(ALT)activities(P＜0.05),and decreased high-density lipoprotein cholesterol(HDL-C)levels(P＜0.05);liver TC,TG,malondialdehyde(MDA),and reactive oxygen species(ROS)levels were significantly increased(P＜0.05),while the activities of catalase(CAT),glutathione peroxidase(GSH-Px),and superoxide dismutase(SOD)were significantly decreased(P＜0.05).The addition of 200 mg/(kg·bw)and 400 mg/(kg·bw)PLP significantly decreased serum TG,TC,LDL-C,and GLU levels,as well as AST and ALT activities in HF fed mice(P＜0.05),and significantly increased HDL-C levels(P＜0.05),significantly reduced liver TC,TG,MDA,and ROS levels(P＜0.05),while enhancing the activities of antioxidant enzymes CAT,GSH-Px,and SOD(P＜0.05).Furthermore,gene expression analysis related to antioxidant and lipid metabolism in mice revealed that PLP significantly upregulated the reduced expression levels of antioxidant genes Nrf2 and HO-1 induced by the HF diet and decreased the expression of lipid metabolism-related genes Lpl,Cyp7α1,and Scd1(P＜0.05).These results suggest that the HF diet can induce lipid metabolism abnormalities and hepatic oxidative stress,leading to liver damage in mice.However,PLP can alleviate oxidative stress and lipid metabolism abnormalities induced by the HF diet,providing a reference for the clinical application of PLP.关键词
车前草多糖/高脂饮食/抗氧化酶活性/氧化应激/脂代谢Key words
plantain polysaccharide/high-fat diet/antioxidant enzyme activity/oxidative stress/lipid metabolism分类
农业科技引用本文复制引用
张力凡,朱俊义..车前草多糖对高脂日粮饲喂小鼠脂代谢和氧化应激的影响[J].中国兽医杂志,2023,59(12):129-134,6.基金项目
吉林省科技发展计划项目(20210202078NC) （20210202078NC）
东北林蛙一体化设施养育技术研究与示范 （）
