中国畜牧兽医2024，Vol.51Issue(1)：23-32,10.DOI:10.16431/j.cnki.1671-7236.2024.01.003
辽宁沈阳株猫泛白细胞减少症病毒VP2基因扩增及生物信息学分析
Amplification and Bioinformatics Analysis of VP2 Gene of a Liaoning Shenyang Strain of Feline Panleukopenia Virus
摘要
Abstract
[Objective]The purpose of this study was to analyze the molecular characteristics of VP2 protein of a Feline panleukopenia virus(FPV)LN3 strain from Shenyang,Liaoning province in 2022 by bioinformatics methods.[Method]FPV colloidal gold test strips were used to detect feces of sick cats with clinical symptoms such as vomiting and diarrhea.The extracted viral DNA from the feces of the diseased cat was amplificated by PCR and sequenced.Sequences were spliced by Seqman software.The VP2 sequence of FPV LN3 strain was subjected to similarity alignment and genetic evolution analysis with FPV and Canine parvovirus(CPV)strains in NCBI.The VP2 protein of FPV LN3 strain was predicted by bioinformatics software,included physical and chemical properties,hydrophilicity and hydrophobicity,transmembrane region,glycosylation site,subcellular localization,protein secondary structure,tertiary structure,etc.[Result]The full length of VP2 gene of FPV LN3 strain was 1 755 bp,which was composed by 584 amino acids.The FPV LN3 strain and other 15 strains registered on GenBank belong to the same major branch,with similarity ranging from 98.9％to 99.7％.FPV LN3 strain and CPV were not belong to the same branch.Bioinformatics software prediction results showed that the VP2 protein of FPV LN3 strain was hydrophilic and had no transmembrane structure.VP2 protein contained 7 potential N-glycosylation sites,86 O-glycosylation sites and 50 phosphorylation sites.The probability of VP2 protein in cytoplasm,nucleus and mitochondria were 43.5％,34.8％and 21.7％,respectively.The randon coil,alpha helix,extended chain and beta turn in the secondary structure of VP2 protein accounted for 61.82％,8.90％,24.32％and 4.97％,respectively,which was consistent with the prediction results of the tertiary structure.There were 20 antigenic epitopes of VP2 protein.[Conclusion]VP 2 was a key gene for genetic variation in FPV.The VP2 protein of FPV LN3 strain was a hydrophilic and non transmembrane stable protein,which contained 20 antigenic epitopes.The results could provide theoretical basis for further study of the function of FPV VP2 protein and the development of new vaccines.关键词
猫泛白细胞减少症病毒/VP2基因/生物信息学/蛋白结构Key words
Feline panleukopenia virus(FPV)/VP2 gene/bioinformatics/protein structure分类
农业科技引用本文复制引用
刘琪,刘正伟,梁琳,张利,郝春晖,李玥,赵福庆..辽宁沈阳株猫泛白细胞减少症病毒VP2基因扩增及生物信息学分析[J].中国畜牧兽医,2024,51(1):23-32,10.基金项目
辽宁省自然科学基金项目-营口联合基金(2021-YKLH-11) （2021-YKLH-11）
辽宁农业职业技术学院2021年院级科研项目(Lnz50) （Lnz50）
