中国畜牧兽医2024，Vol.51Issue(1)：74-85,12.DOI:10.16431/j.cnki.1671-7236.2024.01.008
罗伊氏乳杆菌替代抗生素对仔猪肠道miRNA表达的影响
Effect of Lactobacillus reuteri Replacing Antibiotics on the Expression of Intestinal miRNA in Piglets
摘要
Abstract
[Objective]This study was conducted to investigate the effects of Lactobacillus reuteri LR1 replacing antibiotics on the expression of miRNA in different intestinal segments of piglets,so as to screen potential miRNA for studying the mechanism of Lactobacillus reuteri LR1 on regulating intestinal function of piglets.[Method]A total of 144 cross-bred(DurocX LandraceX Yorkshire)weaned piglets with similar body weight at 21 days old were selected and randomly divided into 3 groups,with 8 replicates in each group and 6 piglets in each replicate.The piglets in control group were fed with the basic diet,the piglets in antibiotic and probiotic groups were fed with the basic diet supplemented with compound antibiotics(100 mg/kg quinoline ethanol+75 mg/kg aureomycin)and 5X1010 CFU Lactobacillus reuteri LR1,respectively.The experiment lasted for 42 days.At the end of the experiment,1 piglet from each replicate was randomly selected and slaughtered,the jejunum tissue was collected for miRNA high-throughput sequencing and analysis.The expression of the selected miRNA in duodenum,jejunum,ileum and colon were detected by Real-time quantitative PCR.[Result]Lactobacillus reuteri LR1 supplementation caused significant changes in miRNA expression,altering the sort of highly expressed miRNA in jejunum.The potential target genes of differentially expressed miRNA were mainly enriched in metabolic related pathways,PI3K-Akt,MAPK,Chemokine and Ras signaling pathways.Real-time quantitative PCR results showed that the expression of differential miRNA in jejunum among 3 groups had the same result with RNA-Seq.Moreover,the expression of up-regulated miRNA showed a relatively consistent trend between control and antibiotic groups,down-regulated miRNA showed a relatively consistent trend between control and probiotic groups,while the highly expressed miRNA in intestine showed a relatively consistent expression trend among 3 groups.[Conclusion]Adding Lactobacillus reuteri LR1 in diet replacing antibiotics could cause changes in the expression of intestinal miRNA.The differentially expressed miRNA screened could provide a foundation for research on the regulating intestinal development and nutrient absorption metabolism by Lactobacillus reuteri LR1 in piglets.关键词
罗伊氏乳杆菌/仔猪/肠道miRNAKey words
Lactobacillus reuteri/piglets/intestinal miRNA分类
农业科技引用本文复制引用
李虹仪,杨雪芬,林泽森,吴晓菲,吕祺欣,余佳莉,张茂..罗伊氏乳杆菌替代抗生素对仔猪肠道miRNA表达的影响[J].中国畜牧兽医,2024,51(1):74-85,12.基金项目
广东省基础与应用基础研究(2021A1515010483) （2021A1515010483）
韶关市支持科研工作者项目(210715124531392、210725204531009) （210715124531392、210725204531009）
