鸡球虫体外培养模型及应用研究进展OACSTPCD

Coccidiosis is a parasitic protozoan disease caused by one or more species of Eimeria parasitizing in the intestinal epithelial cells of chickens.The disease quickly leads to weight loss,malnutrition,intestinal damage,etc.,and has a high economic impact on the poultry industry worldwide.The life cycle of coccidia includes a switch from asexual to sexual stages and is often restricted to a single host.Current research mainly focused on cell biology,protein expression and transport mechanisms in different life stages,host cell infection,host-parasite interactions,new anticoccidial drug screens,etc.Given the diversity of research questions and the requirement of reduce and replace animal experiments,establishing efficient in vitro culture models has become essential for coccidia biological characteristics,anti-coccidia vaccines and drugs study.In this review,the author summarized the in vitro culture model of the poultry parasite coccidia and its application in drug screening,including the chicken embryo culture model,two-dimensional culture models(primary cell culture model,immortalized cell line culture model)and 3D organoid culture model.Breakthroughs in culture techniques have opened new avenues for research on the life cycle stages of coccidia and new intervention strategies and also provide a reference for in-depth understanding of the pathogenic mechanism of coccidia and the development of the anti-coccidia drugs.