中国畜牧兽医2024，Vol.51Issue(1)：292-301,10.DOI:10.16431/j.cnki.1671-7236.2024.01.029
鸡球虫体外培养模型及应用研究进展
Research Progress on Chicken Coccidia in vitro Culture Models and Application
摘要
Abstract
Coccidiosis is a parasitic protozoan disease caused by one or more species of Eimeria parasitizing in the intestinal epithelial cells of chickens.The disease quickly leads to weight loss,malnutrition,intestinal damage,etc.,and has a high economic impact on the poultry industry worldwide.The life cycle of coccidia includes a switch from asexual to sexual stages and is often restricted to a single host.Current research mainly focused on cell biology,protein expression and transport mechanisms in different life stages,host cell infection,host-parasite interactions,new anticoccidial drug screens,etc.Given the diversity of research questions and the requirement of reduce and replace animal experiments,establishing efficient in vitro culture models has become essential for coccidia biological characteristics,anti-coccidia vaccines and drugs study.In this review,the author summarized the in vitro culture model of the poultry parasite coccidia and its application in drug screening,including the chicken embryo culture model,two-dimensional culture models(primary cell culture model,immortalized cell line culture model)and 3D organoid culture model.Breakthroughs in culture techniques have opened new avenues for research on the life cycle stages of coccidia and new intervention strategies and also provide a reference for in-depth understanding of the pathogenic mechanism of coccidia and the development of the anti-coccidia drugs.关键词
鸡/艾美耳球虫/体外培养模型Key words
chicken/Eimeria/in vitro culture model分类
农业科技引用本文复制引用
潘晓婷,胡俊菁,宋勇乐,白银山,孙铭飞,李娟,陈兵,刘俊杰,戚南山,廖申权,吕敏娜,林栩慧,蔡海明..鸡球虫体外培养模型及应用研究进展[J].中国畜牧兽医,2024,51(1):292-301,10.基金项目
广东省农业科学院协同创新中心项目(XTXM202202) （XTXM202202）
广东省科技计划项目(2021B1212050021) （2021B1212050021）
猪禽种业全国重点实验室开放课题(2022GZ07) （2022GZ07）
科技创新战略专项资金(高水平农科院建设)(202110TD、202122TD、R2020PY-JC001、R2019YJ-YB3010、R2020PY-JG013、R2020QD-048、R2021PY-QY007和R2023PY-JG018) （高水平农科院建设）
