中国药房2024，Vol.35Issue(2)：140-144,5.DOI:10.6039/j.issn.1001-0408.2024.02.03
健脾化浊调脂颗粒的化学成分及入血成分分析
Identification of chemical components and components migrating to the blood of Jianpi huazhuo tiaozhi granules
摘要
Abstract
OBJECTIVE To analyze the chemical components and components migrating to the blood of Jianpi huazhuo tiaozhi granules(JHTG).METHODS SD rats were divided into a control group and a medication group,with 6 rats in each group.The medication group was given JHTG 3 mL.Sixty minutes after medication,the serum samples of the 2 groups were collected,and the chemical components and components migrating to the blood of JHTG were separated by ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)and mass spectrometry data were collected.Combined with the overall scheme of UNIFI natural products,based on the 6400 natural product theory mass spectrometry database,the structure was analyzed and confirmed by literature review and reference substance comparison.RESULTS&CONCLUSIONS A total of 130 components were identified from JHTG,including 3 in Codonopsis Radix,13 in Nelumbinis Folium,15 in Poria,5 in Atractylodis Macrocephalae Rhizoma,9 in Citri Reticulatae Pericarpium,1 in Coicis Semen,19 in Alisma Rhizoma,24 in Salviae Miltiorrhizae Radix et Rhizoma,7 in Hordei Fructus Germinatus,24 in Crataegi Fructus,2 in Amomi Fructus,and 3 in Aucklandiae Radix.In addition,quercetin and quercetin-3-O-β-D-glucopyranoside,kaempferol and citric acid may originate from Nelumbinis Folium or Crataegi Fructus,while oleanolic acid may originate from Poria or Crataegi Fructus.By comparing the reference substances,8 components were finally determined(pachymic acid,atractylenolide Ⅱ,alisol A,alisol B,alantolactone,bornyl acetate,salvianolic acid A,salvianolic acid C).A total of 72 prototype components such as quercetin and kaempferol were identified,mainly including flavonoids,terpenoids,lignans and phenolic acids.A total of 11 metabolites such as dehydroanonaine and 16-O-acetylpachymic acid were identified,mainly terpenoids.Metabolic pathways include phase Ⅰ metabolic reactions such as dehydrogenation and dehydroxylation,and phase Ⅱ metabolic reactions such as methylation and acetylation.关键词
健脾化浊调脂颗粒/化学成分/入血成分/超高效液相色谱-串联质谱技术Key words
Jianpi huazhuo tiaozhi granules/chemical components/components migrating to the blood/UPLC-MS/MS分类
医药卫生引用本文复制引用
王逸然,刘中勇..健脾化浊调脂颗粒的化学成分及入血成分分析[J].中国药房,2024,35(2):140-144,5.基金项目
国家自然科学基金项目(No.81960849) （No.81960849）
国家中医药管理局高水平中医药重点学科建设项目(NATCM's Project of High-level Construction of Key TCM Disciplines)(No.zyyzdxk-2023113) （NATCM's Project of High-level Construction of Key TCM Disciplines）
全国名老中医药专家传承工作室建设项目(No.国中医药人教函[2022]75号) （No.国中医药人教函[2022]75号）
江西中医药大学校级研究生创新专项资金立项资助项目(No.JZYC23S50) （No.JZYC23S50）
