中国药房2024，Vol.35Issue(2)：145-149,5.DOI:10.6039/j.issn.1001-0408.2024.02.04
基于GABA能神经系统探究淫羊藿次苷Ⅱ的抗抑郁作用及机制
Exploration of antidepressant effect and mechanism of icariside Ⅱ based on GABAergic nervous system
摘要
Abstract
OBJECTIVE To explore the antidepressant effect and potential mechanism of icariside Ⅱ(ICS Ⅱ)based on the GABAergic nervous system.METHODS The male Kunming mice were randomly divided into a control group(group C,10 mice)and a modeling group(50 mice).The depression model was induced by chronic unpredictable mild stress(CUMS)method in the modeling group.After 21 days of stimulation,the rats of modeling group were randomly divided into depression model group(NS group),positive control group[ECS group,oxalate escitalopram 15 mg/(kg•d)]and ICS Ⅱ low-dose,medium-dose and high-dose groups[ICS Ⅱ-L group,ICS Ⅱ-M group,ICS Ⅱ-H group;ICS Ⅱ 10,20,30 mg/(kg•d)],with 10 mice in each group.Administration groups were given relevant medicine intragastrically,once a day,for 14 consecutive days.The sugar water preference rate,total exercise distance,immobility time in tail suspension and forced swimming experiments were detected in each group.The morphology of neurons and Nissl bodies in the hippocampal CA3 region were observed;the contents of γ-aminobutyric acid(GABA)and glutamic acid(Glu),GABA/Glu ratio,and the expressions of GABAergic nervous system-related proteins(GABA A receptor α1,GABA B receptor 1,vesicular GABA transporter,glutamate decarboxylase 67,GABA membranal transporter 3)were detected in hippocampus.RESULTS Compared with group C,the sugar water preference rate and the total exercise distance significantly reduced in NS group,while the values of immobility time in the tail suspension test and forced swimming test were significantly prolonged(P<0.05).The morphology of neurons in the CA3 area of the hippocampus was irregular and the Nissl bodies were reduced,with a significant decrease in the number of structurally intact neurons(P<0.05);the content of Glu was significantly increased,while the content of GABA,GABA/Glu ratio,and the expressions of GABAergic nervous system-related proteins were significantly decreased(P<0.05).Compared with NS group,depression behavior in each administration group was improved,and the above indexes were mostly reversed(P<0.05).CONCLUSIONS ICS Ⅱ can improve depression behavior of depression model mice.The mechanisms may be associated with regulating the balance of GABA and Glu,increasing the synthesis,transport and release of GABA,and regulating the expressions of GABA-related receptors,so as to improve GABAergic nervous system.关键词
淫羊藿次苷Ⅱ/抑郁/慢性不可预知温和应激/γ氨基丁酸能神经系统Key words
icariside Ⅱ/depression/chronic unpredictable mild stress/GABAergic nervous system分类
医药卫生引用本文复制引用
熊庭旺,张珏,孙成新,尹彩霞,陈犀,陈发菊..基于GABA能神经系统探究淫羊藿次苷Ⅱ的抗抑郁作用及机制[J].中国药房,2024,35(2):145-149,5.基金项目
国家自然科学基金项目(No.31960204) （No.31960204）
遵义市科技计划项目[No.遵市科合HZ字(2021)221号,遵市科合HZ字(2021)214号,遵市科合HZ字(2022)159号] （2021）
