中国药房2024，Vol.35Issue(2)：150-154,5.DOI:10.6039/j.issn.1001-0408.2024.02.05
光皮木瓜特征图谱及3种黄酮类成分含量测定研究
Study on characteristic chromatogram of Chaenomeles sinensis and content determination of 3 flavones
摘要
Abstract
OBJECTIVE To establish the characteristic chromatogram of Chaenomeles sinensis,determine the contents of rutin,hyperin and quercitrin,and to identify C.sinensis and C.speciosa.METHODS HPLC method was performed on Agilent 5 TC-C18 column,with acetonitrile-0.2%formic acid solution as the mobile phase for gradient elution,at the flow rate of 1.0 mL/min.The column temperature was 30℃.The detection wavelength was 330 nm in characteristic chromatogram and 350 nm in content determination.The characteristic chromatogram of C.sinensis was established and similarity was evaluated by the Similarity Evaluation System for Chromatographic Fingerprint of TCM(2012 edition).Hierarchical cluster analysis of 15 batches of C.sinensis(S1-S15)was performed by using SPSS 23.0 software.The contents of 3 flavones in 15 batches of C.sinensis and 7 batches of C.speciosa(S16-S22)were determined,while their characteristic chromatograms were compared.RESULTS The similarities of the characteristic chromatogram for 15 batches of C.sinensis ranged from 0.783 to 0.969,and 11 characteristic peaks were confirmed.Four constituents were identified as chlorogenic acid,rutin,hyperin and quercitrin.The medicinal materials in 15 batches of C.sinensis could be divided into 2 categories:S5-S8 were one category,and the others belonged to one category.The characteristic chromatogram of C.sinensis was obviously different from C.speciosa.The contents of rutin,hyperin and quercitrin in 15 batches of C.sinensis were 48.99-294.45,3.49-102.55,31.98-149.49 μg/g,respectively.The content of rutin in C.speciosa was lower than that in C.sinensis.None of hyperin(except for S20)and quercitrin were detected in C.speciosa.CONCLUSIONS The characteristic chromatogram and the method for content determination of 3 flavones in C.sinensis are established successfully and can be used for the quality control of C.sinensis and its identification from C.speciosa.关键词
光皮木瓜/黄酮/特征图谱/高效液相色谱法Key words
Chaenomeles sinensis/flavones/characteristic chromatogram/HPLC分类
医药卫生引用本文复制引用
朱田密,陈树和,严劲松,王新桂,段雨晴,杨孝义..光皮木瓜特征图谱及3种黄酮类成分含量测定研究[J].中国药房,2024,35(2):150-154,5.基金项目
湖北省科技重大专项课题(No.2020ACA007-01) （No.2020ACA007-01）
