中国药房2024，Vol.35Issue(2)：166-171,6.DOI:10.6039/j.issn.1001-0408.2024.02.08
甘草甜素对大鼠幽门螺杆菌相关性胃炎的改善作用及机制
Improvement effects of glycyrrhizin on Helicobacter pylori-associated gastritis in rats and its mechanism
摘要
Abstract
OBJECTIVE To investigate the improvement effects of glycyrrhizin(GL)on Helicobacter pylori(HP)-associated gastritis in rats and its mechanism.METHODS HP-associated gastritis rat model was induced by inoculating with 1×109 cfu/mL HP.The model rats were randomly divided into model group,positive control group(HP standard quadruple group),GL low-dose,medium-dose and high-dose groups(5,20,50 mg/kg),with 12 rats in each group.Another 12 healthy rats were selected as normal control group.Except the normal control group and model group were given constant volume of normal saline intragastrically,the other groups were given corresponding drugs intragastrically,once a day,for 30 consecutive days.After administration,rats received 13C urea breath test,and delta-over-baseline(DOB)was recorded;the pathological and cellular morphological changes of gastric mucosa in rats were observed,and pathological scoring was performed;the levels of interleukin-8(IL-8),IL-1β,tumor necrosis factor-α(TNF-α),reactive oxygen species(ROS)and malondialdehyde(MDA)were detected in gastric mucosa of rats;mRNA expressions of high mobility group box-1 protein(HMGB1)and nuclear factor-κ-B(NF-κB),relative expressions of nitric oxide synthases(iNOS)and HMGB1,the phosphorylation level of NF-κB p65 were also detected in rats.RESULTS Compared with normal control group,the DOB value,histopathological score of gastric mucosa,the levels of IL-8,IL-1β,TNF-α,ROS and MDA,relative expressions of HMGB1 and NF-κB mRNA,relative expressions of iNOS and HMGB1 protein and the phosphorylation level of NF-κB p65 were all increased significantly in model group(P＜0.05);the epithelial cells of gastric mucosa in rats were incomplete in structure and decreased in the number,with an increase in cell fragments and vacuoles,and significant cell pyknosis.Compared with model group,the changes of the above indexes in GL groups and positive control group were significantly reversed(P＜0.05);the changes in the above indicators in the GL high-dose group were more significant than GL low-dose and medium-dose groups(P＜0.05);the pathological changes of gastric mucosal cells in rats had all improved.CONCLUSIONS GL may inhibit inflammation and oxidative stress by inhibiting the activation of HMGB1/NF-κB pathway,thus relieving HP-induced gastric mucosal injury.关键词
甘草甜素/高迁移率族蛋白B1/核因子κB/幽门螺杆菌相关性胃炎/胃黏膜损伤Key words
glycyrrhizin/high mobility group box 1 protein/nuclear transcription factor-κB/Helicobacter pylori-associated gastritis/gastric mucosal injury分类
医药卫生引用本文复制引用
刘誉华,刘莲,汪九重,黄丹,周素芳,肖欢智,安祯祥..甘草甜素对大鼠幽门螺杆菌相关性胃炎的改善作用及机制[J].中国药房,2024,35(2):166-171,6.基金项目
贵州省科技计划项目(No.黔科合支撑[2020]4Y077号) （No.黔科合支撑[2020]4Y077号）
贵州省卫生健康委科学技术基金项目(No.gzwkj2023-139) （No.gzwkj2023-139）
贵州中医药大学国家与省级科技创新人才团队培育项目(No.贵中医TD合字[2022]005号) （No.贵中医TD合字[2022]005号）
