中国药房2024，Vol.35Issue(2)：172-178,7.DOI:10.6039/j.issn.1001-0408.2024.02.09
止得咳颗粒在大鼠体内的代谢产物研究
Study on metabolites derived from Zhideke granules in rats in vivo
摘要
Abstract
OBJECTIVE To analyze the metabolites of Zhideke granules and speculate its metabolic pathway in rats in vivo.METHODS Male SD rats were randomly divided into blank group and administration group(Zhideke granules,9.45 g/kg);they were given ultrapure water or relevant medicine,twice a day,every 6-8 h,for 3 consecutive days.Serum,urine and feces samples of rats were collected,and their metabolites were identified by UPLC-Q-Exactive-MS technique after intragastric administration of Zhideke granules;their metabolic pathways were speculated.RESULTS After intragastric administration of Zhideke granules,16 prototype components(i.g.irisflorentin,baicalin,chlorogenic acid)and 11 metabolites(i.g.hydration products of kaempferol or luteolin,methylation products of chlorogenic acid,and hydroxylation products of baicalin)were identified in serum,urine and feces of rats.Among them,8 prototype components and 4 metabolites were identified in serum samples;10 prototype components and 7 metabolites were identified in urine samples;8 prototype components and 5 metabolites were identified in the fecal samples.CONCLUSIONS The metabolites of Zhideke granules in rats mainly include baicalin,irisflorentin,chlorogenic acid,and the main metabolic pathways included methylation,hydroxylation,glucuronidation.关键词
止得咳颗粒/超高效液相色谱-四极杆-静电场轨道阱高分辨质谱技术/代谢产物/代谢途径/大鼠Key words
Zhideke granules/UPLC-Q-Exactive-MS/metabolites/metabolic pathway/rat分类
药学引用本文复制引用
梁洁,赵立春,陈日兰,郑飘雪,陈辉华,黄春燕,梁炎丽,陆春莲,谢晶晶,马玉明,彭嘉文..止得咳颗粒在大鼠体内的代谢产物研究[J].中国药房,2024,35(2):172-178,7.基金项目
广西创新驱动发展专项(科技重大专项)(No.桂科AA17202046) （科技重大专项）
广西壮族自治区民族药资源与应用工程研究中心项目(No.桂发改高技函[2020]2605号) （No.桂发改高技函[2020]2605号）
广西中医药重点学科(壮药学)项目(No.GZXK-Z-20-64) （壮药学）
广西高等学校千名中青年骨干教师培育计划项目(No.桂教人[2019]5号) （No.桂教人[2019]5号）
广西中医药大学第三批"歧黄工程"高层次人才团队培育项目 （）
