中国药房2024，Vol.35Issue(2)：186-191,6.DOI:10.6039/j.issn.1001-0408.2024.02.11
银杏叶提取物对糖尿病肾病模型小鼠肾脏炎症的抑制作用及机制
Inhibitory effects of Ginkgo biloba extract on renal inflammation in diabetic nephropathy model mice and its mechanism
摘要
Abstract
OBJECTIVE To investigate the inhibitory effects of Ginkgo biloba extract(GBE)on renal inflammation in diabetic nephropathy(DN)model mice,and its potential mechanism.METHODS KK/Ay mice were fed with high fat and high sugar to induce DN model.They were divided into model group,positive control group[metformin 200 mg/(kg·d)],GBE low-dose and high-dose groups[100,200 mg/(kg·d)],with 6 mice in each group.Six C57BL/6J mice were fed with a regular diet as the control group.Administration groups were given relevant liquid intragastrically,control group and model group were given constant volume of normal saline intragastrically,once a day,for 8 consecutive weeks.The body weight,fasting blood glucose,24-hour food intake,24-hour urine output,monocyte chemoattractant protein-1(MCP-1),interleukin-12(IL-12),IL-10,advanced glycation end products(AGEs),blood urea nitrogen(BUN)and serum creatinine(Scr)of mice were measured,and the ratio of bilateral kidneys to body weight was also calculated.The pathological injury and fibrotic changes of the renal cortex were observed,and the expressions of macrophage polarization marker proteins[type M1:inducible nitric oxide synthase(iNOS);type M2:arginase-1(Arg-1)]and AGEs-the receptor of advanced glycation end products(RAGE)/Ras homolog gene family member A(RhoA)/Rho-associated coiled-coil forming protein kinase(ROCK)signaling pathway-related proteins were determined in renal cortex.RESULTS Compared with the model group,the symptoms such as renal cortical hyperplasia,vacuoles,infiltration of inflammatory cells,and renal cortical fibrosis had been improved in GBE low-dose and high-dose groups;body weight,serum level of IL-10,the expression of Arg-1 in the renal cortex were significantly higher than model group(P＜0.01);fasting blood glucose,24-hour food intake,24-hour urine output,serum levels of MCP-1,IL-12,BUN,Scr and AGEs,the ratio of bilateral kidneys to body weight,renal injury score,the proportion of renal interstitial fibrosis,the protein expressions of iNOS,RAGE,RhoA and ROCK1(except for GBE low-dose group)in renal cortex were significantly lower than model group(P＜0.01).CONCLUSIONS GBE could improve kidney damage and alleviate inflammatory response in DN model mice,the mechanism of which may be related to inhibiting the AGEs-RAGE/RhoA/ROCK signaling pathway and regulating macrophage polarization.关键词
银杏叶提取物/糖尿病肾病/炎症反应/巨噬细胞极化/晚期糖基化终末产物-晚期糖基化终末产物受体/Ras同源基因家族成员A/Rho相关螺旋卷曲蛋白激酶信号通路Key words
Ginkgo biloba extract/diabetic nephropathy/inflammation/macrophage polarization/AGEs-RAGE/RhoA/ROCK signaling pathway分类
医药卫生引用本文复制引用
陈璟,杨小艺,陈静,单鑫,汪洁,许惠琴,吕志阳..银杏叶提取物对糖尿病肾病模型小鼠肾脏炎症的抑制作用及机制[J].中国药房,2024,35(2):186-191,6.基金项目
江苏高校"青蓝工程"培养对象项目(No.苏教师函[2021]11号) （No.苏教师函[2021]11号）
江苏省中医药科技发展计划项目(No.YB2020108) （No.YB2020108）
江苏省高等学校基础科学(自然科学)研究面上项目(No.22KJB360014) （自然科学）
江苏省大学生创新创业训练计划立项项目(No.202313981005Y) （No.202313981005Y）
泰州市科技支撑计划(社会发展)项目(No.SSF20220004) （社会发展）
