中国药房2024，Vol.35Issue(2)：198-203,6.DOI:10.6039/j.issn.1001-0408.2024.02.13
松果菊苷对尿毒症大鼠肾损伤的影响及机制
Effects of echinacoside on renal injury in uremia rats and its mechanism
摘要
Abstract
OBJECTIVE To investigate the effects of echinacoside(ECH)on renal injury in uremia(URE)rats and its mechanism.METHODS URE model of the rat was established by 5/6 nephrectomy.Successfully modeled rats were grouped into uremia group(URE group),ECH low-dose[10 mg/(kg·d)]group,ECH medium-dose[20 mg/(kg·d)]group,ECH high-dose[40 mg/(kg·d)]group,ECH high-dose+anisomycin[p38 mitogen-activated protein kinase(p38 MAPK)pathway activator]group[ECH-H+Ani group,40 mg/(kg·d)ECH +2 mg/(kg·d)anisomycin],with a sham operation group,12 mice in each group.Each drug group was given corresponding ECH intragastrically,while ECH-H+Ani group was further injected with anisomycin via the tail vein,once a day,for 8 consecutive weeks.The serum levels of tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),IL-6,blood urea nitrogen(BUN),β2-microglobulin(β2-MG),serum creatinine(Scr),neutrophil gelatinase-associated lipocalin(NGAL),kidney injury molecule-1(KIM-1),cystatin C(Cys-C)and 24 h urine protein(24 h UP)as well as the levels of malondialdehyde(MDA)and superoxide dismutase(SOD)activity in renal tissue were all detected;pathological changes of renal tissue were observed;the rate of positive expression of α-smooth muscle protein(α-SMA)and E-cadherin,and the phosphorylation of p38 MAPK and nuclear factor-κB(NF-κB)p65 were determined in renal tissue of rats.RESULTS Compared with URE group,glomerular swelling,damage and necrosis of renal tubular epithelial cells and inflammatory cell infiltration were relieved significantly in ECH groups.The renal injury score,levels of TNF-α,IL-1β,IL-6,BUN,Scr,β2-MG,24 h UP,NGAL,KIM-1,Cys-C and MDA,the positive expression rate of α-SMA in renal tissue,the phosphorylation of p38 MAPK and NF-κB p65 were decreased in dose-dependent manner,while SOD activity and the positive expression rate of E-cadherin were obviously increased in dose-dependent manner(P＜0.05).Anisomycin significantly attenuated the improvement effect of high-dose ECH on renal injury in URE rats(P＜0.05).CONCLUSIONS ECH may inhibit inflammation and oxidative stress,enhance renal function,and improve renal injury in uremic rats by inhibiting the activation of p38 MAPK/NF-κB signaling pathway.关键词
松果菊苷/尿毒症/肾损伤/p38丝裂原活化蛋白激酶/核因子κB信号通路Key words
echinacoside/uremia/renal injury/p38 mitogen-activated protein kinase/nuclear factor-κB signaling pathway分类
医药卫生引用本文复制引用
熊玮,彭斌,高智..松果菊苷对尿毒症大鼠肾损伤的影响及机制[J].中国药房,2024,35(2):198-203,6.基金项目
武汉市医学科研项目(No.WZ20C16) （No.WZ20C16）
