中国医科大学学报2024，Vol.53Issue(2)：97-101,120,6.DOI:10.12007/j.issn.0258-4646.2024.02.001
子痫前期胎盘组织中神经型Wiskott-Alrdich综合征蛋白的表达及其意义
Expression and significance of N-WASP in placentas with preeclampsia
摘要
Abstract
Objective To study the expression and clinical significance of neural Wiskott-Alrdich syndrome protein(N-WASP)in pla-centas with preeclampsia.Methods This study included a total of 65 pregnant women:15 in the early-onset preeclampsia group,15 in the early-onset control group,15 in the late-onset preeclampsia group,and 20 in the late-onset control group.Real-time fluorescence quan-titative PCR(RT-qPCR)was used to detect the relative expression of N-WASP mRNA in placental tissues.Western blotting and immu-nohistochemistry were used to detect the expression and position of N-WASP protein in placental tissues from each group.Results RT-qPCR revealed significantly lower N-WASP mRNA expression levels in the placental tissue of the early-onset preeclampsia group compared to those in the early-onset control group(0.50±0.19 vs.0.93±0.73,P＜0.05).The N-WASP mRNA expression levels in late-onset preeclampsia placenta were significantly lower than those in the late-onset control group(0.83±0.34 vs.1.15±0.34,P＜0.05).Western blotting revealed significantly lower N-WASP protein expression in the placental tissue of early-onset preeclampsia compared to that in the early-onset control group(0.35±0.17 vs.0.72±0.21,P＜0.05).The N-WASP protein expression in late-onset preeclampsia placenta was significantly lower than that in the late-onset control group(0.39±0.16 vs.0.76±0.20,P＜0.05).The N-WASP mRNA expression in the placenta negatively correlated with the occurrence of early-onset(r =-0.37,P = 0.042)and late-onset preeclampsia(r =-0.39,P = 0.019).Immunohistochemistry revealed that N-WASP protein was localized in the cytoplasm of syncytiotrophoblasts,cytotrophoblasts,villous stromal cells,and vascular endothelial cells.Conclusion The low expression of N-WASP may be closely associated with preeclampsia.关键词
神经型Wiskott-Alrdich综合征蛋白/子痫前期/滋养细胞Key words
neural Wiskott-Alrdich syndrome protein/preeclampsia/trophoblast分类
医药卫生引用本文复制引用
张硕,葛云鹏,王婷婷,沈鸿飞,李佳钋,宋贵玉,乔宠..子痫前期胎盘组织中神经型Wiskott-Alrdich综合征蛋白的表达及其意义[J].中国医科大学学报,2024,53(2):97-101,120,6.基金项目
国家自然科学基金(81370735,81771610) （81370735,81771610）
百千万人才工程领军人才项目(XLYC2005008) （XLYC2005008）
辽宁省民生科技计划联合计划(2021JH2/10300123) （2021JH2/10300123）
沈阳市科技计划(20-205-4-004) （20-205-4-004）
