中国医科大学学报2024，Vol.53Issue(2)：108-113,6.DOI:10.12007/j.issn.0258-4646.2024.02.003
高盐饮食导致小鼠卵巢组织线粒体功能异常
High salt diet leads to abnormal mitochondrial function in mouse ovarian tissue
摘要
Abstract
Objective To analyze the effect of a high salt diet on ovarian mitochondrial function.Methods Twenty female ICR mice were randomly divided into a normal salt diet(NSD)group and a high salt diet(HSD)group(n = 10 each).The NSD group was given a normal salt diet and the HSD group was given an 8%NaCl diet for 4 weeks.A high salt-treated cell model was established by inducing COV-434 cells cultured in vitro with NaCl.Western blotting was used to detect the protein expression of superoxide dismutase(SOD)and ComplexesⅠ-Ⅴ.The activity of SOD and succinate dehydrogenase(SDH)was detected kinetically.A chemiluminescence assay was used to detect adenosine triphosphate(ATP)levels.Results Compared with the NSD,the HSD significantly reduced the expression level of ComplexⅠin ovarian mitochondria(P＜0.01),significantly increased the expression level of ComplexⅤ(P＜0.05),and significantly reduced the activity of SDH and content of ATP(P＜0.01).The expression level of ComplexesⅠandⅡdecreased significantly(P＜0.05),expression level of ComplexⅤ increased significantly(P＜0.05),activity of SDH decreased significantly(P＜0.01),and content of ATP was insufficient(P＜0.01)in COV-434 cells cultured under high salt conditions.Conclusion High salt can lead to mitochondrial dys-function in the mouse ovary,such as imbalanced oxidative homeostasis,changed expression level of electron transport chain complexes,blocked tricarboxylic acid cycle,and insufficient ATP level.关键词
高盐饮食/小鼠卵巢/线粒体功能障碍/电子传递链/琥珀酸脱氢酶Key words
high salt diet/mouse ovary/mitochondrial dysfunction/electronic transfer chain/succinate dehydrogenase分类
医药卫生引用本文复制引用
侯恩太,顾晓阳,倪士峰,李新宇,乔杰..高盐饮食导致小鼠卵巢组织线粒体功能异常[J].中国医科大学学报,2024,53(2):108-113,6.基金项目
国家自然科学基金(82103106) （82103106）
