中国医科大学学报2024，Vol.53Issue(2)：142-148,7.DOI:10.12007/j.issn.0258-4646.2024.02.009
水飞蓟素通过调控miR-124-3p/WEE1轴影响胶质瘤细胞恶性生长的机制研究
Mechanism of silymarin on malignant growth of glioma cells by regulating miR-124-3p/WEE1 axis
摘要
Abstract
Objective To investigate the impact of silymarin(SM)on the malignant growth of glioma cells and the regulatory mechanism on the miR-124-3p/WEE1 axis.Methods Glioma U87 cells were grouped into control,SM low,medium,and high concentration groups,and SM high concentration + miR-124-3p inhibitor group(SM high + miR-124-3p inhibitor group).CCK-8 was used to measure the proli-feration rate of cells;Transwell® assay was applied to assay the migration and invasion of cells;cell cycle progression was detected by flow cytometry;Western blotting was applied to measure the expression of cyclin D1 and apoptosis-related proteins;the levels of miR-124-3p and WEE1 mRNA were determined by qRT-PCR;and a luciferase activity test was applied to verify the targeting relationship between miR-124-3p and WEE1;in addition,the establishment,administration,and analysis of a NOD/SCID mouse model of intracranial trans-planted tumor were conducted.Results Compared with the control group,the cell proliferation,the numbers of migrating and invading cells,the expression of cyclin D1,and the level of WEE1 mRNA in the various SM treatment groups decreased,the number of cells in G0/G1 phase,the expression of cleaved caspase-8,cleaved caspase-9,cleaved caspase-3 and miR-124-3p increased(P＜0.05);furthermore,transfection of miR-124-3p inhibitor reversed the inhibitory effect of SM on the malignant behavior of glioma cells.In vivo experiments with mice showed that the weights and volumes of tumors in the SM treatment group were lower than those in the model group(P＜0.05),and there was no discernible change in the weight of the mice(P＞0.05).Conclusion SM can inhibit the malignant growth of glioma cells by upregulating miR-124-3p and downregulating WEE1.关键词
水飞蓟素/胶质瘤/miR-124-3p/WEE1轴/恶性生长Key words
silymarin/glioma/miR-124-3p/WEE1 axis/malignant growth分类
医药卫生引用本文复制引用
刘明,刘熙鹏,李淳,张秀峰,曹兵,乔建新,王雪..水飞蓟素通过调控miR-124-3p/WEE1轴影响胶质瘤细胞恶性生长的机制研究[J].中国医科大学学报,2024,53(2):142-148,7.基金项目
河北省2019年度医学科学研究课题(20190883) （20190883）
张家口市科学技术局2020年市级科技计划自筹经费项目(2021115D) （2021115D）
