中国药理学通报2024，Vol.40Issue(1)：38-45,8.DOI:10.12360/CPB202309018
槲皮素对微重力引起骨髓间充质干细胞衰老的影响研究
Influence of quercetin on aging of bone marrow mesenchymal stem cells induced by microgravity
摘要
Abstract
Aim To investigate the effect of quercetin on the aging model of bone marrow mesenchymal stem cells established under microgravity.Methods Using 3D gyroscope,a aging model of bone marrow mesen-chymal stem cells was constructed,and after receiving quercetin and microgravity treatment,the anti-aging effect of the quercetin was evaluated by detecting relat-ed proteins and oxidation indexes.Results Compared to the control group,the expressions of age-related pro-teins p21,p16,p53 and RB in the microgravity group significantly increased,while the expressions of cyclin D1 and lamin B1 significantly decreased,with statisti-cal significance(P＜0.05).In the microgravity group,mitochondrial membrane potential significantly decreased(P＜0.05),ROS accumulation significantly increased(P＜0.05),SOD content significantly de-creased and MDA content significantly increased(P＜0.05).Compared to the microgravity group,the ex-pressions of age-related proteins p21,p16,p53 and RB in the quercetin group significantly decreased,while the expressions of cyclin D1 and lamin B1 signif-icantly increased,with statistical significance(P＜0.05).In the quercetin group,mitochondrial mem-brane potential significantly increased(P＜0.05),ROS accumulation significantly decreased(P＜0.05),SOD content significantly increased and MDA content significantly decreased(P＜0.05).Conclusions Quercetin can resist oxidation,protect mitochondrial function and normal cell cycle,thus delaying the aging of bone marrow mesenchymal stem cells induced by mi-crogravity.关键词
微重力/衰老/骨髓间充质干细胞/槲皮素/氧化应激/lamin B1/线粒体Key words
microgravity/aging/bone marrow stromal cells/quercetin/oxidative stress/lamin B1/mito-chondrion分类
医药卫生引用本文复制引用
杨玉田,轩莹莹,高玉海,王龙飞,唐汉琴,马智慧,李亮,武艺,陈克明..槲皮素对微重力引起骨髓间充质干细胞衰老的影响研究[J].中国药理学通报,2024,40(1):38-45,8.基金项目
国家自然科学基金面上项目(No 81770879) （No 81770879）
甘肃省自然科学基金项目(No 22JR11RA013) （No 22JR11RA013）
甘肃省青年科技基金计划(22JR5RA020) （22JR5RA020）
中国人民解放军联勤保障部队第九四○医院实验室培育项目(No 2021yxky081) （No 2021yxky081）
兰州市科技计划项目(No 2023-1-8) （No 2023-1-8）
