槲皮素对微重力引起骨髓间充质干细胞衰老的影响研究OACSTPCD

Aim To investigate the effect of quercetin on the aging model of bone marrow mesenchymal stem cells established under microgravity.Methods Using 3D gyroscope,a aging model of bone marrow mesen-chymal stem cells was constructed,and after receiving quercetin and microgravity treatment,the anti-aging effect of the quercetin was evaluated by detecting relat-ed proteins and oxidation indexes.Results Compared to the control group,the expressions of age-related pro-teins p21,p16,p53 and RB in the microgravity group significantly increased,while the expressions of cyclin D1 and lamin B1 significantly decreased,with statisti-cal significance(P＜0.05).In the microgravity group,mitochondrial membrane potential significantly decreased(P＜0.05),ROS accumulation significantly increased(P＜0.05),SOD content significantly de-creased and MDA content significantly increased(P＜0.05).Compared to the microgravity group,the ex-pressions of age-related proteins p21,p16,p53 and RB in the quercetin group significantly decreased,while the expressions of cyclin D1 and lamin B1 signif-icantly increased,with statistical significance(P＜0.05).In the quercetin group,mitochondrial mem-brane potential significantly increased(P＜0.05),ROS accumulation significantly decreased(P＜0.05),SOD content significantly increased and MDA content significantly decreased(P＜0.05).Conclusions Quercetin can resist oxidation,protect mitochondrial function and normal cell cycle,thus delaying the aging of bone marrow mesenchymal stem cells induced by mi-crogravity.