中国药理学通报2024，Vol.40Issue(1)：70-75,6.DOI:10.12360/CPB202308023
豨莶草提取物对脑缺血/再灌注大鼠的神经保护作用
The neuroprotective effects of Herba siegesbeckiae extract on cerebral ischemia/reperfusion in rats
摘要
Abstract
Aim To study the neuroprotective effects of Herba siegesbeckiae extract on cerebral ischemia/reperfusion rats and its mechanism.Methods Sixty SD rats were randomly divided into model group,low,middle and high dose groups of Herba siegesbeckiae,and Sham operation group,and the drug was given con-tinuously for seven days.The degree of neurologic im-pairment was evaluated by mNSS,and the infarct vol-ume was measured by MRI.The number of Nissl-posi-tive cells was detected by Nissl staining,and the apop-tosis was accessed by Tunel staining.Furthermore,the expression of Bax,Bcl-2 and NeuN was observed by Western blot,and the expression of NeuN was detected by immunofluorescence staining.The expression of IL-1β,TNF-α and IL-6 mRNA was performed by RT-qPCR.Results The mNSS score and the volume of ischemic cerebral infarction in the model group were significantly increased,and Herba siegesbeckiae extract treatment significantly decreased the mNSS score and infarct volume(P＜0.05,P＜0.01).Herba sieges-beckiae extract could increase the number of Nissl-pos-itive cells and the expression of NeuN(P＜0.01),and reduce the number of Tunel-positive cells(P＜0.01).Western blot showed that Herba siegesbeckiae extract inhibited the expression of Bax,increased Bcl-2 and NeuN in ischemic brain tissue(P＜0.01).RT-qPCR showed that Herba siegesbeckiae extract inhibited the expression of IL-1β,TNF-α.and IL-6 mRNA in the is-chemic brain tissue(P＜0.01).Conclusions Herba siegesbeckiae extract can reduce the cerebral infarction volume,improve the neurological function damage,in-hibit the apoptosis of nerve cells and the expression of inflammatory factors and promote the expression of Ne-uN,there by exerting protective effects on MCAO rats.关键词
豨莶草提取物/脑缺血/再灌注/凋亡/NeuN/mNSS评分/神经保护Key words
Herba siegesbeckiae extract/cerebral is-chemia/reperfusion/apoptosis/NeuN/mNSS score/neuroprotection分类
医药卫生引用本文复制引用
吴慧玲,吴青青,陈景泉,周彬彬,余正双,杨泽霖,赖文芳,洪桂祝..豨莶草提取物对脑缺血/再灌注大鼠的神经保护作用[J].中国药理学通报,2024,40(1):70-75,6.基金项目
国家自然科学基金资助项目(No 82174001,81973503) （No 82174001,81973503）
福建省自然科学基金项目(No 2021J02014,2020J01727) （No 2021J02014,2020J01727）
