中国药理学通报2024，Vol.40Issue(1)：76-82,7.DOI:10.12360/CPB202306068
基于TLR4/NF-κB信号通路探究红花黄色素对不同月龄APP/PS1小鼠学习记忆能力的影响
Effect of safflower yellow on learning and memory ability of APP/PS1 mice at different months based on TLR4/NF-κB signaling pathway
摘要
Abstract
Aim To investigate the effect of safflower yellow(SY)on learning and memory ability of APP/PS1 mice at different disease stages,and to explore the mechanism of SY anti-Alzheimer's disease by using 3-,6-and 9-month-old APP/PS1 transgenic mice as experimental animal models.Methods Behavioral experiments were conducted to observe the effects of SY on learning and memory of APP/PS1 mice of differ-ent months.ELISA was used to detect the effect of SY on the expression of inflammatory factors in cortex of mice of different months.Western blot was used to de-tect the microglia activation marker protein,and its mechanism of action was further analyzed.Results SY could enhance the learning and memory ability of mice aged 3,6 and 9 months,reduce the content of IL-6 and increase the content of TGF-β1 in brain tis-sue,up-regulate the expression levels of arginase-1(arg-1)and triggering receptor expressed on myeloid cells 2(tREM2)in brain tissue of mice of different months,and down-regulate the expression levels of in-ducible nitric oxide synthase(iNOS),Toll-like recep-tors 4(tlr4)and nuclear factor-kappa B(nf-KB).Conclusions Compared with 3-and 9-month-old mice,SY is the most effective in improving learning memory in 6-month-old APP/PS1 mice.SY inhibits TLR4/NF-κB pathway activation by inducing TREM2 expression in brain tissue of APP/PS1 transgenic mice,promotes microglia phenotype shift to anti-inflammatory phenotype,reduces chronic neuroinflammatory re-sponse,and improves learning memory in APP/PS1 mice at all months of age.关键词
红花黄色素/阿尔茨海默病/小胶质细胞/神经炎症/学习记忆/炎症因子Key words
safflower yellow/Alzheimer's disease/microglia/neuroinflammation/learning memory/in-flammatory factor分类
医药卫生引用本文复制引用
张梦玉,郑艳杰,叶红霞,王春辉,胡艳丽..基于TLR4/NF-κB信号通路探究红花黄色素对不同月龄APP/PS1小鼠学习记忆能力的影响[J].中国药理学通报,2024,40(1):76-82,7.基金项目
国家自然科学基金资助项目(No 81960665) （No 81960665）
