中国药理学通报2024，Vol.40Issue(1)：99-105,7.DOI:10.12360/CPB202308045
二烯丙基二硫增强DJ-1过表达人胃癌SGC7901细胞对5-FU的敏感性
Diallyl disulfide augments sensitivity of DJ-1 overexpressed human gastric cells to 5-FU
摘要
Abstract
Aim To investigate whether diallyl disul-fide(DADS)augments the sensitivity of DJ-1(pro-tein/nucleic acid deglycase)overexpressed human gas-tric SGC7901 cells to 5-FU(5-fluorouracil).Methods The experimental groups include control group,DADS group,VCR(vincristine)group,VCR+DADS group,DJ-1 group,DJ-1+DADS group.MTT was used to analyze the effect of DADS on 5-FU(5-fluorou-racil)induced proliferation inhibition.Flow cytometry was performed to examine the effect of DADS on cell apoptosis.RT-PCR,Western blot,and immunofluo-rescence were used for determine the effect of DADS on the drug resistance associated gene expression.Results DADS enhanced the proliferation inhibitory effect of 5-FU on DJ-1 overexpressed cells and VCR resistant cells.DADS could induce apoptosis in VCR-resistant cells.DADS downregulated the expression of DJ-1 while inducing apoptosis in DJ-1 overexpressed cells.DJ-1 overexpression upregulated the expression of P-gp(P-glycoprotein),Bcl-2,and XIAP(X-linked inhibi-tor of apoptosis protein),downregulated the expression of caspase-3.DADS decreased the expression of P-gp,Bcl-2,and XIAP,while increased the expression of caspase-3 in DJ-1 overexpressed cells and VCR-resist-ant cells.Conclusions DADS can augment the sensi-tivity of DJ-1 overexpressed cells to 5-FU,which is re-lated to its antagonism against DJ-1 mediated upregula-tion of P-gp,Bcl-2,XIAP,and downregulation of caspase-3.关键词
二烯丙基二硫/DJ-1/P-糖蛋白/胃癌细胞/耐药/5-氟尿嘧啶Key words
diallyl disulfide/DJ-1/P-glycoprotein/gastric cancer cells/drug resistance/5-fluorouracil分类
医药卫生引用本文复制引用
寻艺,夏红,李志敏,刘芳,苏琦,苏波..二烯丙基二硫增强DJ-1过表达人胃癌SGC7901细胞对5-FU的敏感性[J].中国药理学通报,2024,40(1):99-105,7.基金项目
国家自然科学基金资助项目(No 81973532) （No 81973532）
湖南省教育厅科学研究项目(No 19C1610,No 23A0316) （No 19C1610,No 23A0316）
南华大学科研基金项目(No 220XNK002) （No 220XNK002）
