中国药理学通报2024，Vol.40Issue(1)：106-113,8.DOI:10.12360/CPB202309059
丹酚酸B抑制HuH-7细胞的Hippo/YAP通路机制研究
Study on mechanism of Hippo/YAP pathway inhibition by salvianolic acid B in HuH-7 cells
摘要
Abstract
Aim To investigate whether salvianolic acid B(Sal B)has inhibitory effect on hepatoma HuH-7 cells and explore whether it works via Hippo/YAP signaling pathway.Methods HuH-7 cells were in-duced by TGF-β1(9 μmol·L-1)or the MST1/2 in-hibitor,XMU-MP-1(5,10 μmol·L-1)and pretrea-ted with Sal B(50 μmol·L-1).CCK8 and EdU as-says were used to evaluate cell proliferation.Wound healing assay was used to detect cell migration.Immu-nofluorescence was used to evaluate the expression and distribution of YAP and TAZ.Western blot was used to detect the levels of protein expression of p-MST1,MST1,p-YAP,YAP,p-TAZ,TAZ,and CTGF.Re-sults The results showed that Sal B inhibited the pro-liferation of TGF-β1 or XMU-MP-1-stimulated HuH-7 cells.At the same time,Sal B suppressed the migra-tion of TGF-β1-stimulated HuH-7 cells.Notably,un-like the promotion of XMU-MP-1 on the migration abil-ity of HepG2 cells,XMU-MP-1 could not significantly promote the migration of HuH-7 cells in this experi-ment.In addition,Sal B increased protein expressions of p-MST1,MST1,p-YAP and p-TAZ,while de-creased the expressions of YAP,TAZ,CTGF.Con-clusions The inhibitory effect of Sal B on HuH-7 cells may be related to the activation of Hippo/YAP signaling pathway.关键词
丹酚酸B/HuH-7/肝癌/Hippo/YAP/TGF-β1/XMU-MP-1Key words
Sal B/HuH-7/HCC/Hippo/YAP/TGF-β1/XMU-MP-1分类
医药卫生引用本文复制引用
余新梅,李利利,张傲,邓洁,杨雁..丹酚酸B抑制HuH-7细胞的Hippo/YAP通路机制研究[J].中国药理学通报,2024,40(1):106-113,8.基金项目
国家自然科学基金资助项目(No 82074073) （No 82074073）
安徽医科大学临床医学专业"早期接触科研"训练计划立项项目(No 2021-ZQKY-64,No 2022-ZQKY-175) （No 2021-ZQKY-64,No 2022-ZQKY-175）
