中国药理学通报2024，Vol.40Issue(1)：125-132,8.DOI:10.12360/CPB202303037
雷公藤多苷纳米粒的制备及其对关节炎大鼠的治疗作用
Preparation of tripterygium glycoside nanoparticles and therapeutic effect on arthritis rats
摘要
Abstract
Aim To prepare tripterygium glycoside nanoparticles and probe into their therapeutic effect on collagen-induced arthritis(CIA)rats.Methods Tripterygium glycosides polyglycoside nanoparticles were prepared by thin film dispersion method and their quality was assessed.The CIA model was established and drug intervention performed.The body weight,toe swelling degree and arthritis index were measured.The pathological changes of the organs,knee and ankle sy-novium were observed.The serum levels of kidney function and inflammatory cytokine expression were de-tected in rats.Results The prepared tripterygium wil-fordii polyglycoside nanoparticles were round particles with uniform distribution and stable properties under e-lectron microscope.Compared with the model group,the swelling of the left and right toes of medication group significantly decreased(P＜0.01),and the ar-thritis index markedly decreased(P＜0.01).Among them,the efficacy of the TG-NPs group was better than that of the TG group.Compared with the normal group,the indexes of heart,spleen,kidney and testis all significantly decreased(P＜0.05,P＜0.01).TG-NPs group had a significantly reduced pathological an-kle-joint injury in knee cartilage and increased apoptot-ic synovial cells.Compared with the model group,the serum levels of ALT and BUN and CRE in TG-NPs group were significantly lower(P＜0.05),and IL-1 β,TNF-α and IL-6 levels decreased significantly(P＜0.05).Conclusions TG-NPs have good therapeu-tic effect on CIA through induction of synovial cell ap-optosis and decrease of the expression of inflammatory cytokines.By intravenous injection of blood circula-tion,slow and controlled release of drugs can be a-chieved,the first pass effect caused by oral drug can be avoided,the viscera toxicity can be reduced,which provides an experimental basis for the development of new nanoagents for the treatment of rheumatoid arthri-tis.关键词
雷公藤多苷/纳米粒/新型给药系统/类风湿关节炎/CIA模型/炎症因子/毒性Key words
tripterygium glycosides/nanoparticles/novel drug delivery system/rheumatoid arthritis/CIA model/inflammatory cytokines/toxicity分类
医药卫生引用本文复制引用
王志荣,李嫚,张振强,闫敏,武香香,曾华辉..雷公藤多苷纳米粒的制备及其对关节炎大鼠的治疗作用[J].中国药理学通报,2024,40(1):125-132,8.基金项目
河南省科技研发计划联合基金(优势学科培育类)-培育项目(No 222301420060) （优势学科培育类）
河南省自然基金面上项目(No 222300420482) （No 222300420482）
河南省优秀青年基金资助项目(No 212300410057) （No 212300410057）
