中国药理学通报2024，Vol.40Issue(1)：139-145,7.DOI:10.12360/CPB202303028
液相色谱-质谱联用技术分析秦巴硒菇提取物活性成分及其治疗慢性粒细胞白血病的网络药理学研究
Analysis of effective components of Agaricus blazei Murill extract by LC-MS and network pharmacology analysis in treatment of chronic myeloid leukemia
摘要
Abstract
Aim To explore the potential targets and related signaling pathways of Agaricus blazei Murill(AbM)extract in the treatment of chronic myeloid leu-kemia(CML)based on liquid chromatography mass spectrometry(LC-MS),network pharmacology,mo-lecular docking,and were further verified by experi-ments in vitro.Methods The active components of AbM extract were retrieved from LC-MS,Swiss Target Prediction database was used to predict related targets,and CML disease target genes were obtained from Gen-eCards and DisGeNET databases.After screening the common targets of drug and CML,the protein-protein interaction network of the common targets was per-formed by STRING,and GO and KEGG enrichment a-nalysis were done by DAVID database.Cytoscape soft-ware was used to construct the network of target pro-tein.Molecular docking was carried out by DockThor,and the Pymol software was used to make a visual pic-ture.The inhibitory effect of AbM extract on leukemia cells K562 was determined by CCK-8 experiment,and the effect of AbM extract on the expression and phos-phorylation level of related proteins was verified by Western blot.Results The prediction results showed that 126 active components of AbM extract,and 172 common targets were collected.KEGG pathway analy-sis results showed that PI3 K/Akt/mTOR signaling pathway might play an important role in the treatment of CML disease.The IC50 of AbM extract in K562 cells 24 and 48 hours was 1.86 and 1.48 mg·mL-1,re-spectively.The protein expression of p-PI3K,p-Akt,and p-mTOR was down-regulated.Conclusions AbM extract can perform synergistic effect in treatment of CML through multi-component,multi-channel,multi-target ways,and the mechanism may be related to the regulation of PI3K/Akt/mTOR signaling pathway.关键词
秦巴硒菇提取物/慢性粒细胞白血病/液相色谱质谱联用/网络药理学/分子对接/实验验证Key words
Agaricus blazei extract/chronic myeloid leukemia/LC-MS/network pharmacology/molecular docking/experimental verification分类
医药卫生引用本文复制引用
王东萍,葛万文,邵晶,孙延庆..液相色谱-质谱联用技术分析秦巴硒菇提取物活性成分及其治疗慢性粒细胞白血病的网络药理学研究[J].中国药理学通报,2024,40(1):139-145,7.基金项目
国冢自然科学基金资助项目(No 81560670) （No 81560670）
甘肃省自然科学基金资助项目(No 20JR10RA376,21JR11RA196) （No 20JR10RA376,21JR11RA196）
甘肃省人民医院国家级科研项目培育计划(19SYPYB-17) （19SYPYB-17）
兰州市科技发展指导性计划项目(No 2020-ZD-56) （No 2020-ZD-56）
