中国药理学与毒理学杂志2024，Vol.38Issue(1)：1-10,10.DOI:10.3867/j.issn.1000-3002.2024.01.001
苯并[a]芘恶性转化16HBE细胞元素组分析及铜与顺铂或长春瑞滨联用对细胞增殖的作用
Element profiles of benzo[a]pyrene malignantly transformed 16HBE cells and joint effects of copper with cisplatin or vinorelbine on cell proliferation
摘要
Abstract
OBJECTIVE To assess the profiles of elements in benzo[a]pyrene(BaP)induced carci-nogenesis,and explore the joint effects of copper with cisplatin or vinorelbine on cell proliferation.METHODS Forty-four elements were measured using an inductively coupled plasma mass spectrometer in 16HBE cells and BaP malignantly transformed 16HBE(T-16HBE-C1)cells.Partial least square was used to validate the robustness of cell classification of elements.Cell viability was measured by MTT assay for copper(0,237,340,487,1000 and 1432 μmol·L-1),cisplatin(0,4.4,6.1,8.6,12.0 and 16.8 μmol·L-1),and vinorelbine(0,3.8,9.8,25.0,40.0 and 64.0 μmol·L-1)to acquire their half maximal inhibitory concentra-tion(IC50).Mixtures of copper and chemotherapeutics were prepared according to the ratio of each IC50.Their joint effects on cell viability were assessed by MTT assay and isobolographic analysis.Inhibition effect of copper(0,50,100,200,400 and 800 μmol·L-1)with IC50 of cisplatin or vinorelbine on prolifera-tion of T-16HBE-C1 cells was also assessed.RESULTS A total of 29 elements were quantified in 16HBE and T-16HBE-C1 cells,among which concentrations of copper,zinc,silver,selenium and rubidium decreased(P<0.05,P<0.01),while those of molybdenum,arsenic,lithium,germanium,strontium,nickel,lanthanum,mercury,iron,and cesium increased(P<0.05,P<0.01)in T-16HBE-C1 cells.Element concen-tration could be used to distinguish T-16HBE-C1 cells from 16HBE cells.Copper concentration-dependently inhibited proliferation of both cells,with a statistically significant lower IC50 of(613±16)μmol·L-1 in 16HBE cells than(776±15)μmol·L-1 in T-16HBE-C1 cells(P<0.01).Mixtures of copper and cisplatin(1∶69.5)or vinorelbine(1∶33.4)could inhibit cell proliferation,and copper had additive effects with cisplatin or vinorelbine.When copper concentration was higher than 400 μmol·L-1,copper combined with IC50 of cisplatin or vinorelbine inhibited cell proliferation of T-16HBE-C1 cells compared with IC50 of cisplatin(11.2 μmol·L-1)or vinorelbine(23.2 μmol·L-1)alone.CONCLUSION Element profiles and correlations can change significantly after 16HBE cells are malignantly transformed by BaP.Copper could inhibit the proliferation of T-16HBE-C1 cells and have additive effects with cisplatin or vinorelbine in higher concentration.关键词
元素组/铜/顺铂/长春瑞滨/苯并[a]芘/细胞增殖/T-16HBE-C1细胞Key words
element/copper/cisplatin/vinorelbine/benzo[a]pyrene/cell proliferation/T-16HBE-C1 cells分类
医药卫生引用本文复制引用
王裕,闫赖赖,付娟玲,郝明媚,陈雯,姚碧云,常兵,赵鹏..苯并[a]芘恶性转化16HBE细胞元素组分析及铜与顺铂或长春瑞滨联用对细胞增殖的作用[J].中国药理学与毒理学杂志,2024,38(1):1-10,10.基金项目
National Natural Science Foundation of China(81370079) （81370079）
National Natural Science Foundation of China(81001253) （81001253）
and Beijing Natural Science Foundation(7132122) 国家自然科学基金(81370079) （7132122）
国家自然科学基金(81001253) （81001253）
北京市自然科学基金(7132122) （7132122）
